{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T21:24:15Z","timestamp":1740173055833,"version":"3.37.3"},"reference-count":41,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["P300P3_155374"],"award-info":[{"award-number":["P300P3_155374"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p>Receptor tyrosine kinase-like orphan receptor 1 (ROR1) is an oncofetal antigen expressed on multiple tumors and has no significant expression on normal human tissues. ROR1 is highly upregulated in chronic lymphocytic leukemia (CLL) B cells. NOD-scid IL2rg<jats:sup>\u2212\/\u2212<\/jats:sup> (NSG) mice engrafted with human CD34<jats:sup>+<\/jats:sup> hematopoietic progenitor cells (huNSG) achieved multilineage human immune cell reconstitution including B cells, T cells, NK cells, and DCs. Like the CLL patients, huNSG mice have abnormally high percentage of CD5-expressing B cells in the periphery. In light of this, we aim to determine whether ROR1 is expressed on huNSG B cells. Using flow cytometry analysis, we found that ROR1 was highly expressed in a proportion of bone marrow, spleen, and blood B cells, which were mostly immature B cells. Transplantation of the oncogene TCL-1-transduced CD34<jats:sup>+<\/jats:sup> cells in neonatal NSG mice did not increase the frequency of ROR1-expressing B cells, but the mouse with the highest engraftment of transduced cells developed a tumor-like lump consisting of a high percentage of ROR1-expressing B cells. This study highlights the potential use of huNSG mice to study B cell malignant diseases and to evaluate immunotherapeutics targeting ROR1.<\/jats:p>","DOI":"10.1155\/2018\/2480931","type":"journal-article","created":{"date-parts":[[2018,4,18]],"date-time":"2018-04-18T19:32:34Z","timestamp":1524079954000},"page":"1-10","source":"Crossref","is-referenced-by-count":0,"title":["Analysis of ROR1 Protein Expression in Mice with Reconstituted Human Immune System Components"],"prefix":"10.1155","volume":"2018","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1545-7228","authenticated-orcid":true,"given":"Carol S.","family":"Leung","sequence":"first","affiliation":[{"name":"Department of Hematology, University College London Cancer Institute, University College London, London, UK"},{"name":"Ludwig Institute for Cancer Research, Nuffield Department of Medicine, University of Oxford, Oxford, 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