{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T00:25:59Z","timestamp":1775175959900,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,11,19]],"date-time":"2018-11-19T00:00:00Z","timestamp":1542585600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012015","name":"KNUST","doi-asserted-by":"crossref","award":["kref-0-2016"],"award-info":[{"award-number":["kref-0-2016"]}],"id":[{"id":"10.13039\/501100012015","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Canadian Journal of Infectious Diseases and Medical Microbiology"],"published-print":{"date-parts":[[2018,11,19]]},"abstract":"<jats:p>Tuberculosis (TB) is a major cause of human mortality particularly in association with the human immunodeficiency virus (HIV). <jats:italic>Nocardia<\/jats:italic> spp. has emerged as an opportunistic infection especially in HIV patients. The high prevalence of TB and HIV coupled with the lack of a definitive laboratory diagnosis for <jats:italic>Nocardia<\/jats:italic> spp. could lead to misdiagnosed pulmonary TB. This study determined the prevalence of pulmonary infections due to <jats:italic>Nocardia<\/jats:italic> spp. and <jats:italic>Mycobacterium tuberculosis<\/jats:italic> in sputum of HIV and non-HIV patients with suspected pulmonary tuberculosis at KATH. A total of sixty sputum samples were obtained from HIV and non-HIV patients with suspected pulmonary tuberculosis. Samples were examined by fluorescence based Ziehl\u2013Neelsen staining, culture, and PCR methods. The prevalence of <jats:italic>Nocardia<\/jats:italic> spp. and <jats:italic>Mycobacterium tuberculosis<\/jats:italic> was 18.3% and 20%, respectively, with the latter having the highest rate among patients aged 21\u201340 years (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.075<\/mml:mn><\/mml:mrow><\/mml:math>). The prevalence of <jats:italic>Nocardia<\/jats:italic> spp. among HIV patients was 90.9% whilst 16.7% of the patients had HIV\/<jats:italic>Nocardia<\/jats:italic> spp. coinfection. Detection of <jats:italic>Mycobacterium tuberculosis<\/jats:italic> by fluorescence-based Ziehl\u2013Neelsen staining, culture, and PCR yielded 9 (15%), 11 (18.3%), and 12 (20%), respectively. There is a high prevalence of nocardiosis especially in HIV patients. PCR is a better diagnostic method that detects both <jats:italic>Nocardia<\/jats:italic> spp. and <jats:italic>Mycobacterium tuberculosis<\/jats:italic> and should be incorporated into routine diagnosis for pulmonary infections.<\/jats:p>","DOI":"10.1155\/2018\/2910198","type":"journal-article","created":{"date-parts":[[2018,11,19]],"date-time":"2018-11-19T23:31:11Z","timestamp":1542670271000},"page":"1-7","source":"Crossref","is-referenced-by-count":11,"title":["Evaluating the Contribution of <i>Nocardia<\/i> spp. and <i>Mycobacterium tuberculosis<\/i> to Pulmonary Infections among HIV and Non-HIV Patients at the Komfo Anokye Teaching Hospital, Ghana"],"prefix":"10.1155","volume":"2018","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5168-4762","authenticated-orcid":true,"given":"Samuel Asamoah","family":"Sakyi","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine, School of Medical Sciences, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwabena Owusu","family":"Danquah","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Technology, Faculty of Allied Health Sciences, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5572-9059","authenticated-orcid":true,"given":"Richard Dadzie","family":"Ephraim","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Technology, School of Allied Health Sciences, University of Cape Coast (UCC), Cape Coast, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony","family":"Enimil","sequence":"additional","affiliation":[{"name":"Department of Child Health, School of Medical Sciences, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Venus","family":"Frimpong","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Technology, Faculty of Allied Health Sciences, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0252-3190","authenticated-orcid":true,"given":"Linda","family":"Ahenkorah Fondjo","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, School of Medical Sciences, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5972-3620","authenticated-orcid":true,"given":"Esther Love","family":"Darkoh","sequence":"additional","affiliation":[{"name":"Department of Theoretical and Applied Biology, College of Science, Kwame Nkrumah University of Science and 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