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The numbers of Foxp3<jats:sup>+<\/jats:sup>Tregs and Th1, Th17, and Th22 cells were measured in 32 AIH patients using flow cytometry. Moreover, a murine model of experimental autoimmune hepatitis (EAH) was also established and used to investigate the function of Treg-Th1-Th17-Th22 cells in disease progression. AIH patients undergoing an active state had significantly decreased numbers of CD3<jats:sup>+<\/jats:sup>CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>+<\/jats:sup>Foxp3<jats:sup>+<\/jats:sup>Tregs and increased numbers of CD3<jats:sup>+<\/jats:sup>CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>\u2212<\/jats:sup>Foxp3<jats:sup>+<\/jats:sup>T, CD3<jats:sup>+<\/jats:sup>CD4<jats:sup>+<\/jats:sup>IFN-<jats:italic>\u03b3<\/jats:italic><jats:sup>+<\/jats:sup>Th1, CD3<jats:sup>+<\/jats:sup>CD4<jats:sup>+<\/jats:sup>IL-17<jats:sup>+<\/jats:sup>Th17, and CD3<jats:sup>+<\/jats:sup>CD4<jats:sup>+<\/jats:sup>IL-2<jats:sup>+<\/jats:sup>Th22 cells as well as higher levels of Th1\/Th17\/Th22-type cytokines compared to AIH patients in remission and HC. Additionally, the numbers of CD3<jats:sup>+<\/jats:sup>CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>+<\/jats:sup>Foxp3<jats:sup>+<\/jats:sup>Tregs were negatively correlated with the numbers of Th1-Th17-Th22 cells. Also, the serum levels of IL-17A and IL-22 were correlated positively with liver injury (ALT\/AST), whereas the serum levels of IL-10 were correlated negatively with hypergammaglobulinaemia (IgG, IgM) in AIH patients. Interestingly, the percentages of spleen Tregs, expression of Foxp3 mRNA, and liver IL-10 levels decreased, whereas the percentages of spleen Th1-Th17-Th22 cells, expression of T-bet\/AHR\/ROR<jats:italic>\u03b3<\/jats:italic>t mRNA, and liver IFN-<jats:italic>\u03b3<\/jats:italic>, IL-17, and IL-22 levels increased in the murine model of EAH. Our findings demonstrated that an imbalance between Tregs and Th1-Th17-Th22 cells might contribute to the pathogenic process of AIH.<\/jats:p>","DOI":"10.1155\/2018\/3753081","type":"journal-article","created":{"date-parts":[[2018,6,27]],"date-time":"2018-06-27T23:34:42Z","timestamp":1530142482000},"page":"1-12","source":"Crossref","is-referenced-by-count":49,"title":["The Imbalance between Foxp3<sup>+<\/sup>Tregs and Th1\/Th17\/Th22 Cells in Patients with Newly Diagnosed Autoimmune Hepatitis"],"prefix":"10.1155","volume":"2018","author":[{"given":"Ma","family":"Liang","sequence":"first","affiliation":[{"name":"Department of Digestive Disease, The First People\u2019s Hospital of Changzhou, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, 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