{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T14:46:05Z","timestamp":1753886765141,"version":"3.41.2"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,7,18]],"date-time":"2018-07-18T00:00:00Z","timestamp":1531872000000},"content-version":"vor","delay-in-days":198,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008306","name":"Wroc\u0142aw Medical University","doi-asserted-by":"crossref","award":["167"],"award-info":[{"award-number":["167"]}],"id":[{"id":"10.13039\/501100008306","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Wroc\u0142aw Centre of Biotechnology","award":["167"],"award-info":[{"award-number":["167"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:p>Colostrum and milk are the initial mammalian nourishment and rich reservoir of essential nutrients for newborn development. Bioactive peptides isolated from natural sources, such as colostrum, serve as endogenous regulators and can be used as alternative therapeutic agents in the treatment of neurodegenerative diseases. One example is the previously unknown NP\u2010POL nonapeptide isolated from Colostrinin. In the present study, we investigated a method of NP\u2010POL nonapeptide isolation using Bio\u2010Gel P2 molecular sieve chromatography. We showed the protective effect of NP\u2010POL on 6\u2010hydroxydopamine\u2010 (6\u2010OHDA\u2010) induced neurotoxicity using rat adrenal pheochromocytoma (PC12 Tet On) cells. Treatment of PC12 cells with NP\u2010POL nonapeptide reduced 6\u2010OHDA\u2010induced apoptosis and caused transient phosphorylation of extracellular signal\u2010regulated kinases (ERK 1\/2), which were shown to promote cell survival. NP\u2010POL nonapeptide also protected neuronal cells against oxidative injury induced by 6\u2010OHDA. These results showed a potential use of NP\u2010POL in the therapy of Parkinson\u2019s disease.<\/jats:p>","DOI":"10.1155\/2018\/3760124","type":"journal-article","created":{"date-parts":[[2018,7,19]],"date-time":"2018-07-19T00:03:12Z","timestamp":1531958592000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Isolation and Characterization of NP\u2010POL Nonapeptide for Possible Therapeutic Use in Parkinson\u2019s Disease"],"prefix":"10.1155","volume":"2018","author":[{"given":"Marta","family":"Lemieszewska","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antoni","family":"Polanowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadeusz","family":"Wilusz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agata","family":"Soko\u0142owska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleksandra","family":"Zambrowicz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katarzyna","family":"Miko\u0142ajewicz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f3zefa","family":"Maca\u0142a","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8985-3592","authenticated-orcid":false,"given":"Joanna","family":"Rymaszewska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5707-9886","authenticated-orcid":false,"given":"Agnieszka","family":"Zab\u0142ocka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,7,18]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)61393-3"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2016.03.038"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16305-8"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms130810478"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/7909380"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2007.10.061"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/314560"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.08-125443"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2013.05.001"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF03033371"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aam9080"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-7091-6842-4_7"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(01)00003-X"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(00)00948-4"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2000.0741605.x"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2006.12.004"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/bmb\/ldn013"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.13750"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2017.09.068"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.2174\/187152711794480483"},{"key":"e_1_2_8_21_2","first-page":"127","article-title":"Review of natural products on Parkinson\u2019s disease pathology","volume":"3","author":"Essa M. M.","year":"2014","journal-title":"Journal of Aging Research & Clinical Practice"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(74)80529-6"},{"key":"e_1_2_8_23_2","doi-asserted-by":"publisher","DOI":"10.2174\/156720510791162377"},{"key":"e_1_2_8_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2010.03.003"},{"key":"e_1_2_8_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00005-012-0187-9"},{"key":"e_1_2_8_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/biof.1174"},{"key":"e_1_2_8_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-015-0192-8"},{"key":"e_1_2_8_28_2","doi-asserted-by":"publisher","DOI":"10.1385\/jmn:17:3:379"},{"key":"e_1_2_8_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90587-2"},{"key":"e_1_2_8_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1967.tb00047.x"},{"key":"e_1_2_8_31_2","first-page":"215","article-title":"Colostrinine: a proline-rich polypeptide from ovine colostrum is a modest cytokine inducer in human leukocytes","volume":"44","author":"Inglot A. D.","year":"1996","journal-title":"Archivum Immunologiae et Therapiae Experimentalis"},{"key":"e_1_2_8_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(83)90303-4"},{"key":"e_1_2_8_33_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf00049a007"},{"key":"e_1_2_8_34_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1996.0292"},{"key":"e_1_2_8_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.3015"},{"key":"e_1_2_8_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idairyj.2007.08.004"},{"key":"e_1_2_8_37_2","doi-asserted-by":"publisher","DOI":"10.1080\/10408690490911756"},{"key":"e_1_2_8_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2012.09.016"},{"key":"e_1_2_8_39_2","doi-asserted-by":"publisher","DOI":"10.1385\/JMN:20:2:125"},{"key":"e_1_2_8_40_2","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.21024"},{"key":"e_1_2_8_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfs139"},{"key":"e_1_2_8_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2013.09.014"},{"key":"e_1_2_8_43_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2001.00304.x"},{"key":"e_1_2_8_44_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02970136"}],"container-title":["Oxidative Medicine and Cellular Longevity"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2018\/3760124.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2018\/3760124.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2018\/3760124","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T14:23:36Z","timestamp":1723040616000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2018\/3760124"}},"subtitle":[],"editor":[{"given":"Sebastien","family":"Talbot","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2018,1]]},"references-count":44,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1]]}},"alternative-id":["10.1155\/2018\/3760124"],"URL":"https:\/\/doi.org\/10.1155\/2018\/3760124","archive":["Portico"],"relation":{},"ISSN":["1942-0900","1942-0994"],"issn-type":[{"type":"print","value":"1942-0900"},{"type":"electronic","value":"1942-0994"}],"subject":[],"published":{"date-parts":[[2018,1]]},"assertion":[{"value":"2018-03-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"3760124"}}