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Gangliosides, sialic acid\u2010containing glycosphingolipids expressed in all cells, are involved in cell recognition, signalling, and membrane stabilization. Their expression is often altered under many pathological and physiological conditions including cell death, proliferation, and differentiation. The aim of this study was to assess the possible role of Hmox1 in ganglioside metabolism in relation to oxidative stress. The content of liver and brain gangliosides, their cellular distribution, and mRNA as well as protein expression of key glycosyltransferases were determined in <jats:italic>Hmox1<\/jats:italic> knockout mice as well as their wild\u2010type littermates. To elucidate the possible underlying mechanisms between Hmox1 and ganglioside metabolism, hepatoblastoma HepG2 and neuroblastoma SH\u2010SY5Y cell lines were used for <jats:italic>in vitro<\/jats:italic> experiments. Mice lacking <jats:italic>Hmox1<\/jats:italic> exhibited a significant increase in concentrations of liver and brain gangliosides and in mRNA expression of the key enzymes of ganglioside metabolism. A marked shift of GM1 ganglioside from the subsinusoidal part of the intracellular compartment into sinusoidal membranes of hepatocytes was shown in <jats:italic>Hmox1<\/jats:italic> knockout mice. Induction of oxidative stress by chenodeoxycholic acid <jats:italic>in vitro<\/jats:italic> resulted in a significant increase in GM3, GM2, and GD1a gangliosides in SH\u2010SY5Y cells and GM3 and GM2 in the HepG2 cell line. These changes were abolished with administration of bilirubin, a potent antioxidant agent. These observations were closely related to oxidative stress\u2010mediated changes in sialyltransferase expression regulated at least partially through the protein kinase C pathway. We conclude that oxidative stress is an important factor modulating synthesis and distribution of gangliosides <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> which might affect ganglioside signalling in higher organisms.<\/jats:p>","DOI":"10.1155\/2018\/3845027","type":"journal-article","created":{"date-parts":[[2018,9,19]],"date-time":"2018-09-19T23:48:22Z","timestamp":1537400902000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Heme Oxygenase\u20101 May Affect Cell Signalling via Modulation of Ganglioside Composition"],"prefix":"10.1155","volume":"2018","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8664-7918","authenticated-orcid":false,"given":"V\u00e1clav","family":"\u0160m\u00edd","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6786-0716","authenticated-orcid":false,"given":"Jakub","family":"\u0160uk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neli","family":"Kachamakova-Trojanowska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jana","family":"Ja\u0161prov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8673-1423","authenticated-orcid":false,"given":"Petra","family":"Val\u00e1\u0161kov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7317-260X","authenticated-orcid":false,"given":"Alicja","family":"J\u00f3zkowicz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f3zef","family":"Dulak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franti\u0161ek","family":"\u0160m\u00edd","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5318-0151","authenticated-orcid":false,"given":"Libor","family":"V\u00edtek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2082-1060","authenticated-orcid":false,"given":"Lucie","family":"Muchov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,9,19]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4906(03)00181-9"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2014.00298"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms16035400"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehv114"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.20.10925"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/473709"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1620\/tjem.216.331"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1177\/15353702-0322805-29"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2012.04.026"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2010.01098.x"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.R800028-JLR200"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2007.10.008"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-4328(94)00131-X"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.nutr.28.061807.155515"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.E600002-JLR200"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1174621"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi200601t"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1080\/10715760701618245"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2010007500092"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1021076220411"},{"key":"e_1_2_9_21_2","doi-asserted-by":"publisher","DOI":"10.33549\/physiolres.932665"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.14712\/fb2016062040148"},{"key":"e_1_2_9_23_2","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/762419"},{"volume-title":"Quantitative Clinical Pathology","year":"1995","author":"Hamilton P. 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