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We previously reported a decrease in the percentage of perforin<jats:sup>+<\/jats:sup> cells of stimulated CD8<jats:sup>+<\/jats:sup> lymphocytes derived from patients with malignant mesothelioma. Therefore, we examined the effects of long-term exposure to asbestos on CD8<jats:sup>+<\/jats:sup> T cell functions by comparing long-term cultures of the human CD8<jats:sup>+<\/jats:sup> T cell line EBT-8 with and without exposure to chrysotile (CH) asbestos as an <jats:italic>in vitro<\/jats:italic> model. Exposure to CH asbestos at 5\u2009<jats:italic>\u03bc<\/jats:italic>g\/ml or 30\u2009<jats:italic>\u03bc<\/jats:italic>g\/ml did not result in a decrease in intracellular granzyme B in EBT-8 cells. In contrast, the percentage of perforin<jats:sup>+<\/jats:sup> cells decreased at both doses of CH exposure. CH exposure at 30\u2009<jats:italic>\u03bc<\/jats:italic>g\/ml did not suppress degranulation following stimulation with antibodies to CD3. Secreted production of IFN-<jats:italic>\u03b3<\/jats:italic> stimulated via CD3 decreased by CH exposure at 30\u2009<jats:italic>\u03bc<\/jats:italic>g\/ml, although the percentage of IFN-<jats:italic>\u03b3<\/jats:italic><jats:sup>+<\/jats:sup> cells induced by PMA\/ionomycin did not decrease. These results indicate that long-term exposure to asbestos can potentially suppress perforin levels and the production of IFN-<jats:italic>\u03b3<\/jats:italic> in human CD8<jats:sup>+<\/jats:sup> T cells.<\/jats:p>","DOI":"10.1155\/2018\/4391731","type":"journal-article","created":{"date-parts":[[2018,10,23]],"date-time":"2018-10-23T20:47:27Z","timestamp":1540327647000},"page":"1-10","source":"Crossref","is-referenced-by-count":5,"title":["Decrease in Intracellular Perforin Levels and IFN-<i>\u03b3<\/i> Production in Human CD8<sup>+<\/sup> T Cell Line following Long-Term Exposure to Asbestos Fibers"],"prefix":"10.1155","volume":"2018","author":[{"given":"Naoko","family":"Kumagai-Takei","sequence":"first","affiliation":[{"name":"Department of Hygiene, Kawasaki Medical School, 701-0192 Kurashiki, 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