{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T14:37:38Z","timestamp":1787495858554,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,12,13]],"date-time":"2018-12-13T00:00:00Z","timestamp":1544659200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Canadian Journal of Infectious Diseases and Medical Microbiology"],"published-print":{"date-parts":[[2018,12,13]]},"abstract":"\n Background<\/jats:italic>\n .\n Klebsiella pneumoniae<\/jats:italic>\n and\n Escherichia coli<\/jats:italic>\n are the major extended-spectrum\n \u03b2<\/jats:italic>\n -lactamase- (ESBL-) producing organisms increasingly isolated as causes of complicated urinary tract infections and remain an important cause of failure of therapy with cephalosporins and have serious infection control consequence.\n Objective<\/jats:italic>\n . To assess the prevalence and antibiotics resistance patterns of ESBL-producing\n Escherichia coli<\/jats:italic>\n and\n Klebsiella pneumoniae<\/jats:italic>\n from community-onset urinary tract infections in Jimma University Specialized hospital, Southwest Ethiopia, 2016.\n Methodology<\/jats:italic>\n . A hospital-based cross-sectional study was conducted, and a total of 342 urine samples were cultured on MacConkey agar for the detection of etiologic agents. Double-disk synergy (DDS) methods were used for detection of ESBL-producing strains. A disc of amoxicillin + clavulanic acid (20\/10\u2009\n \u00b5<\/jats:italic>\n g) was placed in the center of the Mueller\u2013Hinton agar plate, and cefotaxime (30\u2009\n \u00b5<\/jats:italic>\n g) and ceftazidime (30\u2009\n \u00b5<\/jats:italic>\n g) were placed at a distance of 20\u2009mm (center to center) from the amoxicillin + clavulanic acid disc. Enhanced inhibition zone of any of the cephalosporin discs on the side facing amoxicillin + clavulanic acid was considered as ESBL producer.\n Results<\/jats:italic>\n . In the current study, ESBL-producing phenotypes were detected in 23% (\n n<\/jats:italic>\n = 17) of urinary isolates, of which\n Escherichia coli<\/jats:italic>\n accounts for 76.5% (\n n<\/jats:italic>\n = 13) and\n K. pneumoniae<\/jats:italic>\n for 23.5% (\n n<\/jats:italic>\n = 4). ESBL-producing phenotypes showed high resistance to cefotaxime (100%), ceftriaxone (100%), and ceftazidime (70.6%), while both ESBL-producing and non-ESBL-producing isolates showed low resistance to amikacin (9.5%), and no resistance was seen with imipenem. In the risk factors analysis, previous antibiotic use more than two cycles in the previous year (odds ratio (OR), 6.238; 95% confidence interval (CI), 1.257\u201330.957;\n p<\/jats:italic>\n = 0.025) and recurrent UTI more than two cycles in the last 6\u2009months or more than three cycles in the last year (OR, 7.356; 95% CI, 1.429\u201337.867;\n p<\/jats:italic>\n = 0.017) were found to be significantly associated with the ESBL-producing groups.\n Conclusion<\/jats:italic>\n . Extended-spectrum\n \u03b2<\/jats:italic>\n -lactamases- (ESBL-)producing strain was detected in urinary tract isolates. The occurrence of multidrug resistance to the third-generation cephalosporins, aminoglycosides, fluoroquinolones, trimethoprim-sulfamethoxazole, and tetracyclines is more common among ESBL producers. Thus, detecting and reporting of ESBL-producing organisms have paramount importance in the clinical decision-making.\n <\/jats:p>","DOI":"10.1155\/2018\/4846159","type":"journal-article","created":{"date-parts":[[2018,12,13]],"date-time":"2018-12-13T18:31:32Z","timestamp":1544725892000},"page":"1-8","source":"Crossref","is-referenced-by-count":67,"title":["Isolation of Extended-Spectrum\n \u03b2<\/i>\n -lactamase- (ESBL-) Producing\n Escherichia coli<\/i>\n and\n Klebsiella pneumoniae<\/i>\n from Patients with Community-Onset Urinary Tract Infections in Jimma University Specialized Hospital, Southwest Ethiopia"],"prefix":"10.1155","volume":"2018","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2700-8216","authenticated-orcid":true,"given":"Mengistu","family":"Abayneh","sequence":"first","affiliation":[{"name":"School of Medical Laboratory Sciences, Mizan-Tepi University, Mizan Aman, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Getnet","family":"Tesfaw","sequence":"additional","affiliation":[{"name":"School of Medical Laboratory Sciences, Institute of Health Sciences, Jimma University, P.O. Box 378, Jimma, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alemseged","family":"Abdissa","sequence":"additional","affiliation":[{"name":"School of Medical Laboratory Sciences, Institute of Health Sciences, Jimma University, P.O. Box 378, Jimma, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki166"},{"key":"2","year":"2009"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0463.2011.02730.x"},{"issue":"8","key":"4","first-page":"1","volume":"9","year":"2011","journal-title":"EFSA Journal"},{"issue":"1","key":"5","first-page":"34","volume":"5","year":"2010","journal-title":"Iranian Journal of Pathology"},{"key":"6","year":"2012"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1128\/aac.01169-07"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-003-1084-2"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1128\/aac.48.1.1-14.2004"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01869.x"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01855.x"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-011-1202-5"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-137-10-200211190-00007"},{"key":"14","year":"2009"},{"key":"15","year":"2014"},{"key":"16","first-page":"268","volume":"18","year":"2011","journal-title":"Clinical Microbiology and Infection"},{"key":"17","year":"2009"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/s1684-1182(10)60031-x"},{"issue":"1","key":"19","first-page":"1","volume":"5","year":"2015","journal-title":"BioPublisher"},{"issue":"7","key":"20","first-page":"321","volume":"4","year":"2015","journal-title":"Global Advanced Research Journal of Medicine and Medical Science"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1186\/1756-0500-3-348"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2006.21.5.816"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1086\/519865"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn293"},{"issue":"6","key":"25","first-page":"1365","volume":"15","year":"2009","journal-title":"Eastern Mediterranean Health Journal"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-12-53"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-011-0128-2"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-011-0132-6"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.168.17.1897"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0051981"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1128\/aac.35.9.1697"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1086\/421094"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.42.3.1089-1094.2004"}],"container-title":["Canadian Journal of Infectious Diseases and Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/cjidmm\/2018\/4846159.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/cjidmm\/2018\/4846159.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/cjidmm\/2018\/4846159.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,7]],"date-time":"2019-04-07T19:31:12Z","timestamp":1554665472000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/cjidmm\/2018\/4846159\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,13]]},"references-count":33,"alternative-id":["4846159","4846159"],"URL":"https:\/\/doi.org\/10.1155\/2018\/4846159","relation":{},"ISSN":["1712-9532","1918-1493"],"issn-type":[{"value":"1712-9532","type":"print"},{"value":"1918-1493","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12,13]]}}}