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Meanwhile, to confirm the relationship between Lin28\/let7 pathway and puberty by overexpression <jats:italic>Lin28a,<\/jats:italic> in the first part of this study, female rats were randomly allocated into untreated controls, the precocious puberty (PP) model group, the PP control group, and the PP + TCM group. Rats on postnatal day 5 were injected danazol to establish the PP model. From days 15 to 35, the rats in the TCM group were given the TCM twice daily. Vaginal opening, sex\u2010related hormones, and body and reproductive organ weights were measured, and the expressions of hypothalamic <jats:italic>Lin28a<\/jats:italic> and <jats:italic>Lin28b<\/jats:italic> mRNA and <jats:italic>let7a<\/jats:italic> and <jats:italic>let7b<\/jats:italic> miRNA were detected. In addition, in the second part, the effects of overexpression of <jats:italic>Lin28a<\/jats:italic> on the vaginal opening time were evaluated. In the two parts of the study, we found that, at the onset of puberty, a decrease in ovary weight, an increase in the serum levels of luteinizing hormone and progesterone, and increased expression levels of hypothalamic <jats:italic>Lin28b<\/jats:italic> mRNA were observed in the PP + TCM group compared to the PP model group. The vaginal opening time was significantly delayed upon overexpression of <jats:italic>Lin28a<\/jats:italic>. Above all, the mechanism by which the TCM treats precocious puberty is thus likely to be associated with inhibition of the hypothalamic Lin28\/let7 signaling pathway and our findings provide in\u2010depth insight into the relationship between the overexpression of <jats:italic>Lin28a<\/jats:italic> gene in the hypothalamus and the onset of puberty.<\/jats:p>","DOI":"10.1155\/2018\/4868045","type":"journal-article","created":{"date-parts":[[2018,6,26]],"date-time":"2018-06-26T23:32:05Z","timestamp":1530055925000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Precocious Puberty and the Lin28\/Let7 Pathway: The Therapeutic Effect of the Nourishing \u201cYin\u201d and Purging \u201cFire\u201d Traditional Chinese Medicine Mixture in a Rat 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