{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T17:37:37Z","timestamp":1772300257383,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006095","name":"Helse S\u00f8r-\u00d8st RHF","doi-asserted-by":"publisher","award":["39570"],"award-info":[{"award-number":["39570"]}],"id":[{"id":"10.13039\/501100006095","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Disease Markers"],"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. After perinatal asphyxia, the cerebellum presents more damage than previously suggested. <jats:italic>Objectives<\/jats:italic>. To explore if the antioxidant N-acetylcysteine amide (NACA) could reduce cerebellar injury after hypoxia-reoxygenation in a neonatal pig model. <jats:italic>Methods<\/jats:italic>. Twenty-four newborn pigs in two intervention groups were exposed to 8% oxygen and hypercapnia, until base excess fell to \u221220\u2009mmol\/l or the mean arterial blood pressure declined to &lt;20\u2009mmHg. After hypoxia, they received either NACA (NACA group, <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>12<\/mml:mn><\/mml:math>) or saline (vehicle-treated group, <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>12<\/mml:mn><\/mml:math>). One sham-operated group (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>5<\/mml:mn><\/mml:math>) served as a control and was not subjected to hypoxia. Observation time after the end of hypoxia was 9.5\u2009hours. <jats:italic>Results<\/jats:italic>. The intranuclear proteolytic activity in Purkinje cells of asphyxiated vehicle-treated pigs was significantly higher than that in sham controls (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mi>p<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.03<\/mml:mn><\/mml:math>). Treatment with NACA was associated with a trend to decreased intranuclear proteolytic activity (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mi>p<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.08<\/mml:mn><\/mml:math>), There were significantly less mutations in the mtDNA of the NACA group compared with the vehicle-treated group, 2.0\u2009\u00d7\u200910<jats:sup>\u22124<\/jats:sup> (\u00b12.0\u2009\u00d7\u200910<jats:sup>\u22124<\/jats:sup>) versus 4.8\u2009\u00d7\u200910<jats:sup>\u22125<\/jats:sup>(\u00b13.6\u2009\u00d7\u200910<jats:sup>\u22124<\/jats:sup>, <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>). <jats:italic>Conclusion<\/jats:italic>. We found a trend to lower proteolytic activity in the core of Purkinje cells and significantly reduced mutation rate of mtDNA in the NACA group, which may indicate a positive effect of NACA after neonatal hypoxia. Measuring the proteolytic activity in the nucleus of Purkinje cells could be used to assess the effect of different neuroprotective substances after perinatal asphyxia.<\/jats:p>","DOI":"10.1155\/2018\/5046372","type":"journal-article","created":{"date-parts":[[2018,1,30]],"date-time":"2018-01-30T18:31:23Z","timestamp":1517337083000},"page":"1-9","source":"Crossref","is-referenced-by-count":6,"title":["Cerebellum Susceptibility to Neonatal Asphyxia: Possible Protective Effects of N-Acetylcysteine Amide"],"prefix":"10.1155","volume":"2018","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2094-6170","authenticated-orcid":true,"given":"T.","family":"Benterud","sequence":"first","affiliation":[{"name":"Department of Pediatric Research, Division of Pediatric and Adolescent Medicine, Oslo University Hospital, Rikshospitalet, Oslo, Norway"},{"name":"Department for Surgical Research, Oslo University Hospital, Rikshospitalet, Oslo, Norway"},{"name":"University of Oslo, Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Manueldas","sequence":"additional","affiliation":[{"name":"Department of Pediatric Research, Division of Pediatric and Adolescent Medicine, Oslo University Hospital, Rikshospitalet, Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Rivera","sequence":"additional","affiliation":[{"name":"Aix Marseille Universit\u00e9, CNRS, NICN, Marseille, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Henckel","sequence":"additional","affiliation":[{"name":"Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, Sweden"},{"name":"Department of Neonatology, Karolinska University Hospital, Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. M.","family":"L\u00f8berg","sequence":"additional","affiliation":[{"name":"University of Oslo, Oslo, Norway"},{"name":"Department of Pathology, Oslo University Hospital, Ullev\u00e5l, University of Oslo, Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Norgren","sequence":"additional","affiliation":[{"name":"Department of Women\u2019s and Children\u2019s Health, Division of Paediatric Endocrinology, Karolinska Institutet, Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. O.","family":"Baumbusch","sequence":"additional","affiliation":[{"name":"Department of Pediatric Research, Division of Pediatric and Adolescent Medicine, Oslo University Hospital, Rikshospitalet, Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4042-6076","authenticated-orcid":true,"given":"R.","family":"Solberg","sequence":"additional","affiliation":[{"name":"Department of Pediatric Research, Division of Pediatric and Adolescent Medicine, Oslo University Hospital, Rikshospitalet, Oslo, Norway"},{"name":"Department for Surgical Research, Oslo University Hospital, Rikshospitalet, Oslo, Norway"},{"name":"Department of Pediatrics, Vestfold Hospital Trust, T\u00f8nsberg, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O. 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