{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T20:29:37Z","timestamp":1740169777646,"version":"3.37.3"},"reference-count":43,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,6,28]],"date-time":"2018-06-28T00:00:00Z","timestamp":1530144000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2018,6,28]]},"abstract":"<jats:p>We evaluated the utility of<jats:italic>Borrelia burgdorferi<\/jats:italic>sensu stricto<jats:italic>(Bb)<\/jats:italic>peptide C6 for serologic confirmation of Ixodid Tick-Borne Borrelioses (ITBB) in Russia. Serum samples (N = 1089) were from erythema migrans (EM) (N = 327) and the EM-free (EMF) patients (N = 115); in some patients, the disease was accompanied by human granulocytic anaplasmosis or tick-borne encephalitis. The sera were investigated by multiplex phosphorescence analysis (PHOSPHAN) for IgM to<jats:italic>Bb<\/jats:italic>C6, recombinant OspC and VlsE proteins, and IgG to C6 from<jats:italic>Bb<\/jats:italic>,<jats:italic>B. garinii<\/jats:italic>(<jats:italic>Bg<\/jats:italic>), and<jats:italic>B. afzelii<\/jats:italic>(<jats:italic>Ba<\/jats:italic>). Detection of<jats:italic>Bb<\/jats:italic>C6 IgM\/IgG provided effective serologic confirmation of ITBB in both EM and EMF patients early after disease onset. In the EM-free patients, however, this test needed to be supplemented with detection of VlsE IgM in convalescent-phase sera due to delay in development of the antibody responses for C6 IgG. In general, positive PHOSPHAN reactions were observed in 81.9% and 86.7% of the EM and EMF patients, respectively, as well as in 59 of 65 (90.8%) patients, whose blood contained<jats:italic>B. burgdorferi<\/jats:italic>sensu lato DNA. Additional detection of IgG to<jats:italic>Bg<\/jats:italic>C6 or<jats:italic>Ba<\/jats:italic>C6 had no significant contribution to serologic diagnosis of ITBB in both patient groups.<\/jats:p>","DOI":"10.1155\/2018\/5291926","type":"journal-article","created":{"date-parts":[[2018,6,28]],"date-time":"2018-06-28T23:38:30Z","timestamp":1530229110000},"page":"1-12","source":"Crossref","is-referenced-by-count":0,"title":["Utility of<i>Borrelia burgdorferi<\/i>sensu stricto C6 Peptide for Serologic Confirmation of Erythema-Free Ixodid Tick-Borne Borrelioses in Russia"],"prefix":"10.1155","volume":"2018","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3377-3731","authenticated-orcid":true,"given":"Vera","family":"Pomelova","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Diagnostics, Department of Biological Microassay, State Research Institute of Biological Engineering, 75\/1 Volokolamskoye Highway, Moscow 125424, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduard","family":"Korenberg","sequence":"additional","affiliation":[{"name":"Department of Infections with Natural Focality, N.F. 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