{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,5]],"date-time":"2025-07-05T16:40:08Z","timestamp":1751733608896,"version":"3.41.0"},"reference-count":34,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,7,5]],"date-time":"2018-07-05T00:00:00Z","timestamp":1530748800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science Foundation, Sri Lanka","award":["RH\/2014\/HS\/04"],"award-info":[{"award-number":["RH\/2014\/HS\/04"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Disease Markers"],"published-print":{"date-parts":[[2018,7,5]]},"abstract":"<jats:p>Potential use of total nitrite plus nitrate (NOx) and nitrite (NO<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>) separately as surrogate markers for serum nitric oxide in severe dengue and their longitudinal changes along with the progression of infection was studied. Deproteinized sera from confirmed dengue fever (DF,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>145<\/mml:mn><\/mml:math>) and dengue hemorrhagic fever (DHF,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>74<\/mml:mn><\/mml:math>) patients on admission\u2014A, critical\u2014C, discharge\u2014D, and convalescence\u2014CON stages and from age-gender matched healthy individuals (HC,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>77<\/mml:mn><\/mml:math>) were taken to assess NO<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>and NOx levels using Griess and modified Griess assays. Serum NOx in DHFA was significantly lower compared to DFA (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.001<\/mml:mn><\/mml:math>). HC had the lowest NOx and NO<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>compared to all patient categories (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.001<\/mml:mn><\/mml:math>) except NO<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>in DF-CON and DHF-CON and NOx in DHF-CON. Serum NOx and NO<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>in DHF patients admitted on fever day 3 (DHFA-3) was significantly lower compared to DFA-3 (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>). Cut-off values of 4.46\u2009<jats:italic>\u03bc<\/jats:italic>M for NOx (91.3% sensitivity and 80.1% specificity) and 1.25\u2009<jats:italic>\u03bc<\/jats:italic>M for NO<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>(75.0% sensitivity and 73.3% specificity) were obtained for day 3 of fever. Serum NOx may be used as potential prognostic marker of DHF in patients presenting with DF in the early stage (on day 3 of fever) of the disease.<\/jats:p>","DOI":"10.1155\/2018\/5328681","type":"journal-article","created":{"date-parts":[[2018,7,5]],"date-time":"2018-07-05T23:36:27Z","timestamp":1530833787000},"page":"1-9","source":"Crossref","is-referenced-by-count":8,"title":["Is Total Serum Nitrite and Nitrate (NOx) Level in Dengue Patients a Potential Prognostic Marker of Dengue Hemorrhagic Fever?"],"prefix":"10.1155","volume":"2018","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8026-3502","authenticated-orcid":true,"given":"Maheshi","family":"Mapalagamage","sequence":"first","affiliation":[{"name":"Institute of Biochemistry Molecular Biology & Biotechnology, University of Colombo, No 90, Cumaratunga Munidasa Mawatha, Colombo 03, Sri 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