{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:55:01Z","timestamp":1790801701923,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,9,10]],"date-time":"2018-09-10T00:00:00Z","timestamp":1536537600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Federal Department of Industry of Russian Federation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2018,9,10]]},"abstract":"<jats:p><jats:italic>Pseudomonas aeruginosa<\/jats:italic>is a cause of high mortality in burn, immunocompromised, and surgery patients. High incidence of antibiotic resistance in this pathogen makes the existent therapy inefficient. Type three secretion system (T3SS) is a leading virulence system of<jats:italic>P. aeruginosa<\/jats:italic>that actively suppresses host resistance and enhances the severity of infection. Innovative therapeutic strategies aiming at inhibition of type three secretion system of<jats:italic>P. aeruginosa<\/jats:italic>are highly attractive, as they may reduce the severity of clinical manifestations and improve antibacterial immune responses. They may also represent an attractive therapy for antibiotic-resistant bacteria. Recently our laboratory developed a new small molecule inhibitor belonging to a class 2,4-disubstituted-4H-<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mo stretchy=\"false\">[<\/mml:mo><mml:mn fontstyle=\"italic\">1,3<\/mml:mn><mml:mo>,<\/mml:mo><mml:mn fontstyle=\"italic\">4<\/mml:mn><mml:mo stretchy=\"false\">]<\/mml:mo><\/mml:math>-thiadiazine-5-ones, Fluorothiazinon (FT), that effectively suppressed T3SS in chlamydia and salmonella<jats:italic>in vitro<\/jats:italic>and<jats:italic>in vivo.<\/jats:italic>In this study, we evaluate the activity of FT towards antibiotic-resistant clinical isolates of<jats:italic>P. aeruginosa<\/jats:italic>expressing T3SS effectors ExoU and ExoS in an airway infection model. We found that FT reduced mortality and bacterial loads and decrease lung pathology and systemic inflammation. In addition, we show that FT inhibits the secretion of ExoT and ExoY, reduced bacteria cytotoxicity, and increased bacteria internalization<jats:italic>in vitro<\/jats:italic>. Overall, FT shows a strong potential as an antibacterial therapy of antibiotic-resistant<jats:italic>P. aeruginosa<\/jats:italic>infection.<\/jats:p>","DOI":"10.1155\/2018\/5810767","type":"journal-article","created":{"date-parts":[[2018,9,10]],"date-time":"2018-09-10T19:30:56Z","timestamp":1536607856000},"page":"1-13","source":"Crossref","is-referenced-by-count":49,"title":["Small Molecule Inhibitor of Type Three Secretion System Belonging to a Class 2,4-disubstituted-4H-[1,3,4]-thiadiazine-5-ones Improves Survival and Decreases Bacterial Loads in an Airway<i>Pseudomonas aeruginosa<\/i>Infection in Mice"],"prefix":"10.1155","volume":"2018","author":[{"given":"Anna B.","family":"Sheremet","sequence":"first","affiliation":[{"name":"Gamaleya Center of Epidemiology and Microbiology, Ministry of Health Russian Federation, 123098, Gamaleya Str. 18, Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naylia 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