{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T14:49:28Z","timestamp":1753886968137,"version":"3.41.2"},"reference-count":53,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,6,13]],"date-time":"2018-06-13T00:00:00Z","timestamp":1528848000000},"content-version":"vor","delay-in-days":163,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81472912"],"award-info":[{"award-number":["81472912"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:p>Herb mixtures are widely used for treatment of the menopausal syndrome long before the hormonal therapy. However, there is insufficient data for herb remedies in treating menopausal syndromes. Here we aim to investigate the effect of ZhenRongDan (ZRD) in balancing female hormones, regulating expression of estrogen receptors (ERs), and preventing organ atrophy in menopausal rats. Rats that underwent bilateral ovariectomy were used in the experiments; the effects of ZRD on serum follicle\u2010stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), and estradiol (E2) levels were observed. Histology of vagina and ERs expression in vagina, uterus, and adrenal gland were also examined. ELISAs were used to analyze the changes of FSH, LH, PRL, and E2 in serum, and the morphological changes of the cervical epithelium cells were observed by Hematoxylin &amp; Eosin (H&amp;E) staining. Immunohistochemistry and western blot were applied to detect estrogen receptors subtypes alpha (ER<jats:italic>\u03b1<\/jats:italic>) and beta (ER<jats:italic>\u03b2<\/jats:italic>) expression in vagina, uterus, and adrenal gland. We found that ZRD could significantly reduce the weight of the adrenal gland and increase the weight of the uterus. It could decrease the release of FSH and LH as well as increasing E2 and PRL levels. Furthermore, ZRD could improve the number of cervical vaginal epithelial cells and increase the thickness of the vaginal wall. And the altered expressions of ER<jats:italic>\u03b1<\/jats:italic> and ER<jats:italic>\u03b2<\/jats:italic> are also restored by ZRD. ZRD could obviously relieve the endocrine disorders, modulate organ atrophy, and restore ER<jats:italic>\u03b1<\/jats:italic> and ER<jats:italic>\u03b2<\/jats:italic> expression in the ovariectomized rat model.<\/jats:p>","DOI":"10.1155\/2018\/5896398","type":"journal-article","created":{"date-parts":[[2018,6,13]],"date-time":"2018-06-13T23:49:13Z","timestamp":1528933753000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Herb Formula ZhenRongDan Balances Sex Hormones, Modulates Organ Atrophy, and Restores ER<i>\u03b1<\/i> and ER<i>\u03b2<\/i> Expressions in Ovariectomized Rats"],"prefix":"10.1155","volume":"2018","author":[{"given":"Xuan","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fangqin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9138-5521","authenticated-orcid":false,"given":"Xiao-Hong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,6,13]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003081-198103000-00020"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.321.7275.1516"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjmed.2005.09.057"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.288.3.321"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2017.06.013"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2016.12.011"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.maturitas.2017.01.013"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.maturitas.2009.11.020"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.19082\/5826"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep17129"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13020-015-0051-z"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-11-137"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.4103\/0976-500X.110868"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11655-011-0931-8"},{"volume-title":"Research on four herbs decoction (Siwu Tang) and its associated prescriptions","year":"2004","author":"Xuehui H.","key":"e_1_2_10_15_2"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/1749-8546-8-5"},{"key":"e_1_2_10_17_2","unstructured":"CFDA Notice on the publication of the fourth batch of OCT drug List 1 2002."},{"key":"e_1_2_10_18_2","first-page":"287","article-title":"Effects of Zhen Rong Dan Mixture on symptoms of menopausal syndrome in mice","volume":"21","author":"Chen B.","year":"2016","journal-title":"Chinese Journal of Clinical Pharmacology and Therapeutics"},{"key":"e_1_2_10_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201700550"},{"key":"e_1_2_10_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2014.03.122"},{"key":"e_1_2_10_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2018.03.023"},{"key":"e_1_2_10_22_2","first-page":"8648","article-title":"Effects of Gengnianchun on learning and memory ability, neurotransmitter, cytokines, and leptin in ovariectomized rats","volume":"8","author":"Rao Y. 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