{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T14:47:40Z","timestamp":1753886860857,"version":"3.41.2"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,11,21]],"date-time":"2018-11-21T00:00:00Z","timestamp":1542758400000},"content-version":"vor","delay-in-days":324,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16K00853"],"award-info":[{"award-number":["16K00853"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:p>Influenza virus infections are a serious public health concern throughout the world. Emergence of viral resistance to the currently approved anti\u2010influenza drugs warrants the development of new antiviral agents. Japanese herbal medicines called Kampo are very commonly used as prescription medication in Japan, and Mao\u2010to is known to be effective against influenza that is caused by oseltamivir\u2010resistant viruses. However, influenza\u2010related death occurs mainly among the elderly, and for patients with hypertension and diabetes, Mao\u2010to may cause these diseases to worsen. Therefore, the exploration of more potent and safe Kampo medicines may be a good strategy for developing new influenza medicines. Here cell\u2010based screening of anti\u2010influenza virus activity for 42 approved Kampo medicines was performed using the drug\u2010repositioning approach. As a result, four Kampo medicines were selected as potent anti\u2010influenza agents against the A\/WSN\/33 strain. It was found that Daio\u2010kanzo\u2010to [50% inhibitory concentration (IC<jats:sub>50<\/jats:sub>) = 10.5 <jats:italic>\u03bc<\/jats:italic>g\/mL; 50% cytotoxic concentration (CC<jats:sub>50<\/jats:sub>) = 71.6 <jats:italic>\u03bc<\/jats:italic>g\/mL; selective index = 6.8] is more effective than Mao\u2010to. Daio\u2010kanzo\u2010to and its constituent Japanese Pharmacopoeia (JP) Rhubarb were also effective against H3N2 and H1N1 subtypes of influenza viruses, including oseltamivir\u2010insensitive\u20102009 pandemic clinical isolates. These data suggest the potential application of Daio\u2010kanzo\u2010to for influenza treatment.<\/jats:p>","DOI":"10.1155\/2018\/6058181","type":"journal-article","created":{"date-parts":[[2018,11,21]],"date-time":"2018-11-21T23:31:26Z","timestamp":1542843086000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Drug\u2010Repositioning Approach for the Discovery of Anti\u2010Influenza Virus Activity of Japanese Herbal (Kampo) Medicines <i>In Vitro<\/i>: Potent High Activity of Daio\u2010Kanzo\u2010To"],"prefix":"10.1155","volume":"2018","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2592-3472","authenticated-orcid":false,"given":"Ken","family":"Watanabe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,11,21]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90452-i"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.1879"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2175"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/518936"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc062989"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(17)33293-2"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02858780"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.61.267"},{"key":"e_1_2_8_9_2","first-page":"128","article-title":"Anti-human immunodeficiency virus activity of oligosaccharides from rooibos tea (Aspalathus linearis) extracts in vitro","volume":"11","author":"Nakano M.","year":"1997","journal-title":"Leukemia"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1177\/1934578X1701200432"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.5582\/ddt.v5.1.26"},{"key":"e_1_2_8_12_2","first-page":"189","article-title":"Anti-influenza activity of Alchemilla mollis extract: possible virucidal activity against influenza virus particles","volume":"7","author":"Makau J. 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The effects of drying and freezing processing on the quality of Rhubarb (Rheum palmatum Rhizome)","volume":"49","author":"Yoneda K.","year":"1995","journal-title":"Journal of Natural Medicines"},{"key":"e_1_2_8_33_2","first-page":"294","article-title":"Studies of Rhei rhizoma (Da-huang ; Dai-o) (II) - The reason Da-huang is processed by heating","volume":"55","author":"Yoshida A.","year":"2001","journal-title":"Journal of Natural Medicines"},{"key":"e_1_2_8_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.35.12.2463"}],"container-title":["Evidence-Based Complementary and Alternative Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2018\/6058181.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2018\/6058181.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2018\/6058181","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T05:24:08Z","timestamp":1722921848000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2018\/6058181"}},"subtitle":[],"editor":[{"given":"I-Min","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2018,1]]},"references-count":34,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1]]}},"alternative-id":["10.1155\/2018\/6058181"],"URL":"https:\/\/doi.org\/10.1155\/2018\/6058181","archive":["Portico"],"relation":{},"ISSN":["1741-427X","1741-4288"],"issn-type":[{"type":"print","value":"1741-427X"},{"type":"electronic","value":"1741-4288"}],"subject":[],"published":{"date-parts":[[2018,1]]},"assertion":[{"value":"2018-06-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"6058181"}}