{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:33:01Z","timestamp":1792787581207,"version":"4.5.0"},"reference-count":58,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ISCIII RETIC REDINREN FEDER Funds","award":["PT13\/0001"],"award-info":[{"award-number":["PT13\/0001"]}]},{"name":"ISCIII RETIC REDINREN FEDER Funds","award":["RD12\/0021\/0033"],"award-info":[{"award-number":["RD12\/0021\/0033"]}]},{"name":"ISCIII RETIC REDINREN FEDER Funds","award":["PI10\/01261"],"award-info":[{"award-number":["PI10\/01261"]}]},{"name":"ISCIII RETIC REDINREN FEDER Funds","award":["PT13\/0001"],"award-info":[{"award-number":["PT13\/0001"]}]},{"name":"ISCIII RETIC REDINREN FEDER Funds","award":["RD12\/0021\/0033"],"award-info":[{"award-number":["RD12\/0021\/0033"]}]},{"name":"ISCIII RETIC REDINREN FEDER Funds","award":["PI10\/01261"],"award-info":[{"award-number":["PI10\/01261"]}]},{"name":"Fondo de Investigaci\u00f3n Sanitaria, Ministerio de Sanidad","award":["PT13\/0001"],"award-info":[{"award-number":["PT13\/0001"]}]},{"name":"Fondo de Investigaci\u00f3n Sanitaria, Ministerio de Sanidad","award":["RD12\/0021\/0033"],"award-info":[{"award-number":["RD12\/0021\/0033"]}]},{"name":"Fondo de Investigaci\u00f3n Sanitaria, Ministerio de Sanidad","award":["PI10\/01261"],"award-info":[{"award-number":["PI10\/01261"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Diabetes Research"],"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p>\n                    Urinary proteome was analyzed and quantified by tandem mass tag (TMT) labeling followed by bioinformatics analysis to study diabetic nephropathy (DN) pathophysiology and to identify biomarkers of a clinical outcome. We included type 2 diabetic normotensive non-obese males with (\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                      <mml:mi>n<\/mml:mi>\n                      <mml:mo>=<\/mml:mo>\n                      <mml:mn>9<\/mml:mn>\n                    <\/mml:math>\n                    ) and without (\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\">\n                      <mml:mi>n<\/mml:mi>\n                      <mml:mo>=<\/mml:mo>\n                      <mml:mn>11<\/mml:mn>\n                    <\/mml:math>\n                    ) incipient DN (microalbuminuria). Sample collection included blood and urine at baseline (control and DN basal) and, in DN patients, after 3 months of losartan treatment (DN treated). Urinary proteome analysis identified 166 differentially abundant proteins between controls and DN patients, 27 comparing DN-treated and DN-basal patients, and 182 between DN-treated patients and controls. The mathematical modeling analysis predicted 80 key proteins involved in DN pathophysiology and 15 in losartan effect, a total of 95 proteins. Out of these 95, 7 are involved in both processes. VCAM-1 and neprilysin stand out of these 7 for being differentially expressed in the urinary proteome. We observed an increase of VCAM-1 urine levels in DN-basal patients compared to diabetic controls and an increase of urinary neprilysin in DN-treated patients with persistent albuminuria; the latter was confirmed by ELISA. Our results point to neprilysin and VCAM-1 as potential candidates in DN pathology and treatment.\n                  <\/jats:p>","DOI":"10.1155\/2018\/6165303","type":"journal-article","created":{"date-parts":[[2018,4,29]],"date-time":"2018-04-29T19:31:35Z","timestamp":1525030295000},"page":"1-12","source":"Crossref","is-referenced-by-count":23,"title":["Urinary Proteome Analysis Identified Neprilysin and VCAM as Proteins Involved in Diabetic Nephropathy"],"prefix":"10.1155","volume":"2018","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8498-2579","authenticated-orcid":true,"given":"Elena","family":"Guill\u00e9n-G\u00f3mez","sequence":"first","affiliation":[{"name":"Molecular Biology Laboratory, Fundaci\u00f3 Puigvert, Barcelona, Spain"},{"name":"Universitat Aut\u00f2noma de Barcelona, Institut d\u2019Investigaci\u00f3 Biom\u00e8dica Sant Pau (IIB Sant Pau), Barcelona, 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Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Calero","sequence":"additional","affiliation":[{"name":"Universitat Aut\u00f2noma de Barcelona, Institut d\u2019Investigaci\u00f3 Biom\u00e8dica Sant Pau (IIB Sant Pau), Barcelona, Spain"},{"name":"Hypertension Unit, Nephrology Department, Fundaci\u00f3 Puigvert, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 A.","family":"Ballar\u00edn","sequence":"additional","affiliation":[{"name":"Universitat Aut\u00f2noma de Barcelona, Institut d\u2019Investigaci\u00f3 Biom\u00e8dica Sant Pau (IIB Sant Pau), Barcelona, Spain"},{"name":"Hypertension Unit, Nephrology Department, Fundaci\u00f3 Puigvert, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7472-2124","authenticated-orcid":true,"given":"Patricia","family":"Fern\u00e1ndez-Llama","sequence":"additional","affiliation":[{"name":"Universitat 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