{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T21:04:11Z","timestamp":1740171851478,"version":"3.37.3"},"reference-count":24,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004427","name":"Universidad de Valpara\u00edso","doi-asserted-by":"publisher","award":["CI 05\/2006"],"award-info":[{"award-number":["CI 05\/2006"]}],"id":[{"id":"10.13039\/501100004427","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Centro de Investigaciones Biom\u00e9dicas","award":["CI 05\/2006"],"award-info":[{"award-number":["CI 05\/2006"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Stem Cells International"],"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p><jats:italic>Purpose<\/jats:italic>.\u00a0Biliary atresia precedes liver cirrhosis and liver transplantation. Amniotic membrane (AM) promotes tissue regeneration, inhibits fibrosis, and reduces inflammation. Here, we test amniotic membrane potential as a therapeutic tool against cholestatic liver fibrosis. <jats:italic>Methods<\/jats:italic>. Three groups of rats were used: sham surgery (SS), bile duct ligature (BDL), and bile duct ligature plus human amniotic membrane (BDL\u2009+\u2009AM). After surgery, animals were sacrificed at different weeks. Biochemical and histopathological analyses of liver tissue were performed. Collagen was expressed as a percentage of total liver tissue area. qPCR was performed to analyse gene expression levels of transforming growth factor-<jats:italic>\u03b2<\/jats:italic>1 (<jats:italic>Tgfb1<\/jats:italic>) and apelin (<jats:italic>Apln<\/jats:italic>). Statistical analysis performed considered <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math> was significant. <jats:italic>Results<\/jats:italic>.\u00a0Groups undergoing BDL developed cholestasis. Biochemical markers from BDL\u2009+\u2009AM group improved compared to BDL group. Ductular reaction, portal fibrosis, and bile plugs were markedly reduced in the BDL\u2009+\u2009AM group compared to BDL group. Collagen area in BDL\u2009+\u2009AM group was statistically decreased compared to BDL group. Finally, expression levels of both <jats:italic>Apln<\/jats:italic> and <jats:italic>Tgfb1<\/jats:italic> mRNA were statistically downregulated in BDL\u2009+\u2009AM group versus BDL group. <jats:italic>Conclusion<\/jats:italic>. AM significantly reduces liver fibrosis in a surgical animal model of cholestasis. Our results suggest that AM may be useful as a therapeutic tool in liver cirrhosis.<\/jats:p>","DOI":"10.1155\/2018\/6169546","type":"journal-article","created":{"date-parts":[[2018,2,25]],"date-time":"2018-02-25T18:32:49Z","timestamp":1519583569000},"page":"1-9","source":"Crossref","is-referenced-by-count":5,"title":["Transplantation of Human Amniotic Membrane over the Liver Surface Reduces Hepatic Fibrosis in a Cholestatic Model in Young Rats"],"prefix":"10.1155","volume":"2018","author":[{"given":"M.","family":"Garrido","sequence":"first","affiliation":[{"name":"Centro de Investigaciones Biom\u00e9dicas, Universidad de Valpara\u00edso, Valpara\u00edso, Chile"},{"name":"Servicio de Cirug\u00eda Pedi\u00e1trica, Hospital Carlos van Buren, Valpara\u00edso, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Escobar","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Biom\u00e9dicas, Universidad de Valpara\u00edso, Valpara\u00edso, 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