{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:50:07Z","timestamp":1790833807045,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,11,19]],"date-time":"2018-11-19T00:00:00Z","timestamp":1542585600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Medical Disciplines Building Project of Shenzhen","award":["SZXJ2018043"],"award-info":[{"award-number":["SZXJ2018043"]}]},{"name":"Key Medical Disciplines Building Project of Shenzhen","award":["20151964"],"award-info":[{"award-number":["20151964"]}]},{"name":"Shenzhen public service platform for clinical drug trials","award":["SZXJ2018043"],"award-info":[{"award-number":["SZXJ2018043"]}]},{"name":"Shenzhen public service platform for clinical drug trials","award":["20151964"],"award-info":[{"award-number":["20151964"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2018,11,19]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Influenza A virus (IAV) has had the highest morbidity globally over the past decade. A growing number of studies indicate that the upper respiratory tract (URT) microbiota plays a key role for respiratory health and that a dysfunctional respiratory microbiota is associated with disease; but the impact of microbiota during influenza is understudied.<jats:italic> Methods<\/jats:italic>. We recruited 180 children, including 121 IAV patients and 59 age-matched healthy children. Nasopharyngeal (NP) and oropharyngeal (OP) swabs were collected to conduct 16S rDNA sequencing and compare microbiota structures in different individuals.<jats:italic> Results<\/jats:italic>. Both NP and OP microbiota in IAV patients differed from those in healthy individuals. The NP dominated genera in IVA patients, such as<jats:italic> Moraxella<\/jats:italic>,<jats:italic> Staphylococcus<\/jats:italic>,<jats:italic> Corynebacterium<\/jats:italic>, and<jats:italic> Dolosigranulum<\/jats:italic>, showed lower abundance than in healthy children. The<jats:italic> Streptococcus <\/jats:italic>significantly enriched in patients\u2019 NP and<jats:italic> Phyllobacterium<\/jats:italic> could be generally detected in patients\u2019 NP microbiota. The most abundant genera in OP microbiota showed a decline tendency in patients, including<jats:italic> Streptococcus, Neisseria<\/jats:italic>, and<jats:italic> Haemophilus.<\/jats:italic> The URT\u2019s bacterial concurrence network changed dramatically in patients. NP and OP samples were clustered into subgroups by different dominant genera; and NP and OP microbiota provided the precise indicators to distinguish IAV patients from healthy children.<jats:italic> Conclusion<\/jats:italic>. This is the first respiratory microbiome analysis on pediatric IAV infection which reveals distinct NP and OP microbiota in influenza patients. It provides a new insight into IAV research from the microecology aspect and promotes the understanding of IAV pathogenesis.<\/jats:p>","DOI":"10.1155\/2018\/6362716","type":"journal-article","created":{"date-parts":[[2018,11,19]],"date-time":"2018-11-19T18:33:30Z","timestamp":1542652410000},"page":"1-9","source":"Crossref","is-referenced-by-count":48,"title":["Distinct Nasopharyngeal and Oropharyngeal Microbiota of Children with Influenza A Virus Compared with Healthy Children"],"prefix":"10.1155","volume":"2018","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8463-688X","authenticated-orcid":true,"given":"Zhixin","family":"Wen","sequence":"first","affiliation":[{"name":"Department of Respiratory Diseases, Shenzhen Children\u2019s Hospital, No. 7019, Yitian Road, Futian District, Shenzhen 518026, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gan","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Respiratory Diseases, Shenzhen 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