{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T14:36:51Z","timestamp":1768833411623,"version":"3.49.0"},"reference-count":42,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,6,28]],"date-time":"2018-06-28T00:00:00Z","timestamp":1530144000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University Medical Centre Hamburg-Eppendorf"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Disease Markers"],"published-print":{"date-parts":[[2018,6,28]]},"abstract":"<jats:p>The underlying mechanisms of ovarian cancer (OvCa) dissemination are still poorly understood, and novel molecular markers for this cancer type are urgently needed. In search of adhesion molecules with prognostic relevance in OvCa, we compared tumors with good outcome (alive\u2009&gt;\u20093 years) and those with poor outcome (dead\u2009&lt;\u20092 years) within data from The Cancer Genome Atlas (TCGA). The carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) turned out as the only gene with differential expression in these groups. In order to further investigation on its role in OvCa, we analyzed CEACAM1 mRNA levels extracted from TCGA microarray data (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>517<\/mml:mn><\/mml:math>) as well as CEACAM1 protein expression by Western blot analysis in a cohort of 242 tumor samples. Further, CEACAM1 localization in tumour tissue was evaluated by immunohistochemistry and CEACAM1 splice variants by RT-PCR in representative tumours. In Kaplan\u2013Meier analysis, high CEACAM1 mRNA levels significantly correlated with longer survival (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>p<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.008<\/mml:mn><\/mml:math>). By Western blot analysis in the second cohort, similar associations of high CEACAM1 protein levels with longer recurrence-free survival (RFS, <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>p<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.035<\/mml:mn><\/mml:math>) and overall survival (OAS, <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mi>p<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.004<\/mml:mn><\/mml:math>) were observed. In multivariate Cox regression analysis including clinical prognostic parameters, CEACAM1 mRNA or protein expression turned out as independent prognostic markers. Stratified survival analysis showed that high CEACAM1 protein expression was prognostic in node-negative tumors (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mi>p<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.045<\/mml:mn><\/mml:math> and <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\"><mml:mi>p<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.0002<\/mml:mn><\/mml:math> for DFS and OAS) but lost prognostic significance in node-positive carcinomas. Similarly, high CEACAM1 mRNA expression did not show prognostic relevance in tumors with lymphatic invasion (L1) but was associated with longer survival in cases without lymphovascular involvement. Further analysis showed a predominance of 4S and 4L isoforms and mostly membraneous CEACAM1 localization in ovarian tumours. Our results suggest that CEACAM1 might be an independent favorable prognostic marker in OvCa, especially in the subgroup of patients with solely intraperitoneal metastasis.<\/jats:p>","DOI":"10.1155\/2018\/6714287","type":"journal-article","created":{"date-parts":[[2018,6,28]],"date-time":"2018-06-28T19:39:31Z","timestamp":1530214771000},"page":"1-10","source":"Crossref","is-referenced-by-count":11,"title":["Prognostic Impact of CEACAM1 in Node-Negative Ovarian Cancer Patients"],"prefix":"10.1155","volume":"2018","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9075-0280","authenticated-orcid":true,"given":"Leticia","family":"Oliveira-Ferrer","sequence":"first","affiliation":[{"name":"Department of Gynecology, University Medical Center Hamburg-Eppendorf, Martinistra\u00dfe 52, 20246 Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roshni","family":"Goswami","sequence":"additional","affiliation":[{"name":"Department of Gynecology, University Medical Center Hamburg-Eppendorf, Martinistra\u00dfe 52, 20246 Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Galatenko","sequence":"additional","affiliation":[{"name":"Moscow State University, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Gynecology, University Medical Center Hamburg-Eppendorf, Martinistra\u00dfe 52, 20246 Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathrin","family":"Eylmann","sequence":"additional","affiliation":[{"name":"Department of Gynecology, University Medical Center Hamburg-Eppendorf, Martinistra\u00dfe 52, 20246 Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Legler","sequence":"additional","affiliation":[{"name":"Department of Gynecology, University Medical Center Hamburg-Eppendorf, Martinistra\u00dfe 52, 20246 Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sascha","family":"K\u00fcrti","sequence":"additional","affiliation":[{"name":"Department of Gynecology, University Medical Center Hamburg-Eppendorf, Martinistra\u00dfe 52, 20246 Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Schmalfeldt","sequence":"additional","affiliation":[{"name":"Department of Gynecology, University Medical Center Hamburg-Eppendorf, Martinistra\u00dfe 52, 20246 Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karin","family":"Milde-Langosch","sequence":"additional","affiliation":[{"name":"Department of Gynecology, University Medical Center Hamburg-Eppendorf, Martinistra\u00dfe 52, 20246 Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"issue":"11","key":"1","first-page":"527","volume":"1","year":"1990","journal-title":"Cell Growth & Differentiation"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.13.2.1273"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(05)80223-2"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1038\/nri1864"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2006.08.008"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63433-5"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cgt.7700055"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.22.10744"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1177\/002215549704500705"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2006.05.020"},{"issue":"1","key":"11","first-page":"249","volume":"29","year":"2009","journal-title":"Anticancer Research"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-013-9444-6"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1080\/2162402x.2017.1328336"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-11-0526"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-14-1752"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2015.00234"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1007\/s00280-014-2662-y"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/j.anndiagpath.2015.10.012"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-013-1489-y"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6604650"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-015-3652-3"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.10.5139"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1038\/nature10166"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1093\/biostatistics\/4.2.249"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1177\/44.1.8543780"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2001.00147.x"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1006\/scbi.1998.0119"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2002.08.067"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/290536"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1202935"},{"issue":"1","key":"31","first-page":"190","volume":"55","year":"1995","journal-title":"Cancer Research"},{"issue":"15","key":"32","first-page":"3431","volume":"56","year":"1996","journal-title":"Cancer Research"},{"issue":"6","key":"33","first-page":"1215","volume":"55","year":"1995","journal-title":"Cancer Research"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2008.136"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.26072"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-013-3460-1"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2014-308781"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.3746"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.17978"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2015.436"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.232711199"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2017.6791"}],"container-title":["Disease Markers"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/dm\/2018\/6714287.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/dm\/2018\/6714287.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/dm\/2018\/6714287.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,6,28]],"date-time":"2018-06-28T19:39:40Z","timestamp":1530214780000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/dm\/2018\/6714287\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6,28]]},"references-count":42,"alternative-id":["6714287","6714287"],"URL":"https:\/\/doi.org\/10.1155\/2018\/6714287","relation":{},"ISSN":["0278-0240","1875-8630"],"issn-type":[{"value":"0278-0240","type":"print"},{"value":"1875-8630","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,6,28]]}}}