{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T21:20:51Z","timestamp":1790371251296,"version":"4.1.0"},"reference-count":18,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["3325\/2013"],"award-info":[{"award-number":["3325\/2013"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["00516-14"],"award-info":[{"award-number":["00516-14"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003758","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa e ao Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico do Maranh\u00e3o","doi-asserted-by":"publisher","award":["3325\/2013"],"award-info":[{"award-number":["3325\/2013"]}],"id":[{"id":"10.13039\/501100003758","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003758","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa e ao Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico do Maranh\u00e3o","doi-asserted-by":"publisher","award":["00516-14"],"award-info":[{"award-number":["00516-14"]}],"id":[{"id":"10.13039\/501100003758","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p>The hydroalcoholic extract of <jats:italic>Punica granatum<\/jats:italic> (pomegranate) leaves was previously demonstrated to be anti-inflammatory in a rat model of lipopolysaccharide- (LPS-) induced acute peritonitis. Here, we investigated the anti-inflammatory effects of the ethyl acetate fraction obtained from the pomegranate leaf hydroalcoholic extract (EAFPg) on the LPS-induced acute lung injury (ALI) mouse model. Male Swiss mice received either EAFPg at different doses or dexamethasone (per os) prior to LPS intranasal instillation. Vehicle-treated mice were used as controls. Animals were culled at 4\u2009h after LPS challenge, and the bronchoalveolar lavage fluid (BALF) and lung samples were collected for analysis. EAFPg and kaempferol effects on NO and cytokine production by LPS-stimulated RAW 264.7 macrophages were also investigated. Pretreatment with EAFPg (100\u2013300\u2009mg\/kg) markedly reduced cell accumulation (specially neutrophils) and collagen deposition in the lungs of ALI mice. The same animals presented with reduced lung and BALF TNF-<jats:italic>\u03b1<\/jats:italic> and IL-1<jats:italic>\u03b2<\/jats:italic> expression in comparison with vehicle controls (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>). Additionally, incubation with either EAFPg or kaempferol (100\u2009<jats:italic>\u03bc<\/jats:italic>g\/ml) reduced NO production and cytokine gene expression in cultured LPS-treated RAW 264.7 macrophages. Overall, these results demonstrate that the prophylactic treatment with EAFPg attenuates acute lung inflammation. We suggest this fraction may be useful in treating ALI.<\/jats:p>","DOI":"10.1155\/2018\/6879183","type":"journal-article","created":{"date-parts":[[2018,2,20]],"date-time":"2018-02-20T18:32:00Z","timestamp":1519151520000},"page":"1-11","source":"Crossref","is-referenced-by-count":14,"title":["<i>Punica granatum<\/i> L. Leaf Extract Attenuates Lung Inflammation in Mice with Acute Lung Injury"],"prefix":"10.1155","volume":"2018","author":[{"given":"Aruan\u00e3 Joaquim Matheus Costa","family":"Rodrigues Pinheiro","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o, Universidade CEUMA, S\u00e3o Lu\u00eds, MA, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o da Rede BIONORTE, Universidade Federal do Amaz\u00f4nas, Manaus, AM, Brazil"},{"name":"Departamento de Farm\u00e1cia, Faculdade Pit\u00e1goras, S\u00e3o Luis, MA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaciara S\u00e1","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o, Universidade CEUMA, S\u00e3o Lu\u00eds, MA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1dylla Wilenna Alves","family":"Dourado","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o, Universidade CEUMA, S\u00e3o Lu\u00eds, MA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo Martins","family":"de Sousa","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o, Universidade CEUMA, S\u00e3o Lu\u00eds, MA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natilene Mesquita","family":"Brito","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Estudos Ambientais, Instituto Federal do Maranh\u00e3o, S\u00e3o Lu\u00eds, MA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanna Karinny","family":"Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Estudos Ambientais, Instituto Federal do Maranh\u00e3o, S\u00e3o Lu\u00eds, MA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisa Cristina Aranha","family":"Batista","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Farmacognosia I, Universidade Federal do Maranh\u00e3o, S\u00e3o Lu\u00eds, MA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joicy Cortez","family":"de S\u00e1","sequence":"additional","affiliation":[{"name":"Departamento de Medicina, Universidade CEUMA, S\u00e3o Lu\u00eds, MA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cinara Regina Arag\u00e3o Vieira","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o, Universidade CEUMA, S\u00e3o Lu\u00eds, MA, Brazil"},{"name":"Centro de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Universidade Federal do Maranh\u00e3o, S\u00e3o Lu\u00eds, MA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth Soares","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o, Universidade CEUMA, S\u00e3o Lu\u00eds, MA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2868-1314","authenticated-orcid":true,"given":"Val\u00e9rio","family":"Monteiro-Neto","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o, Universidade CEUMA, S\u00e3o Lu\u00eds, MA, Brazil"},{"name":"Centro de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Universidade Federal do Maranh\u00e3o, S\u00e3o Lu\u00eds, MA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee Ann","family":"Campbell","sequence":"additional","affiliation":[{"name":"Department of Global Health, University of Washington, Seattle, WA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia Maria Wiziack","family":"Zago","sequence":"additional","affiliation":[{"name":"Departamento de Odontologia, Universidade CEUMA, S\u00e3o Lu\u00eds, MA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7696-0882","authenticated-orcid":true,"given":"Lidio Gon\u00e7alves","family":"Lima-Neto","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o, Universidade CEUMA, S\u00e3o Lu\u00eds, MA, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o da Rede BIONORTE, Universidade Federal do Amaz\u00f4nas, Manaus, AM, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0036-1596952"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00561"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201610-2035CI"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1055\/s-0035-1546169"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00444.2015"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2006.09.006"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-967167"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1055\/s-0042-108856"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.5799"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-013-0659-3"},{"issue":"3","key":"11","first-page":"449","volume":"28","year":"2014","journal-title":"Journal of Biological Regulators and Homeostatic Agents"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2013.05.040"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2012.07.004"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2012.07.007"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/164202"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(03)13413-7"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(15)60367-1"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2011.02.024"}],"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2018\/6879183.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2018\/6879183.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2018\/6879183.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,2,20]],"date-time":"2018-02-20T18:32:03Z","timestamp":1519151523000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/jir\/2018\/6879183\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018]]},"references-count":18,"alternative-id":["6879183","6879183"],"URL":"https:\/\/doi.org\/10.1155\/2018\/6879183","relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"value":"2314-8861","type":"print"},{"value":"2314-7156","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018]]}}}