{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T20:29:36Z","timestamp":1740169776899,"version":"3.37.3"},"reference-count":25,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81460529","81660578","81260476"],"award-info":[{"award-number":["81460529","81660578","81260476"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81460529","81660578","81260476"],"award-info":[{"award-number":["81460529","81660578","81260476"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81460529","81660578","81260476"],"award-info":[{"award-number":["81460529","81660578","81260476"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p>To investigate the mechanism of azalomycin <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:msub><mml:mrow><mml:mtext>F<\/mml:mtext><\/mml:mrow><mml:mrow><mml:mtext>5a<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math> against methicillin-resistant<jats:italic> Staphylococcus aureus <\/jats:italic>(MRSA), the conductivity of MRSA suspension and the adenylate kinase activity of MRSA culture were determined with the intervention of azalomycin <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mrow><mml:msub><mml:mrow><mml:mtext>F<\/mml:mtext><\/mml:mrow><mml:mrow><mml:mtext>5a<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>, which were significantly increased compared to those of blank controls. This inferred that azalomycin <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mrow><mml:msub><mml:mrow><mml:mtext>F<\/mml:mtext><\/mml:mrow><mml:mrow><mml:mtext>5a<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math> could lead to the leakage of cellular substances possibly by increasing permeability to kill MRSA. As phospholipid bilayer was mainly responsible for cell-membrane permeability, the interaction between azalomycin <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mrow><mml:msub><mml:mrow><mml:mtext>F<\/mml:mtext><\/mml:mrow><mml:mrow><mml:mtext>5a<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math> and cell-membrane lipids was further researched by determining the anti-MRSA activities of azalomycin <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mrow><mml:msub><mml:mrow><mml:mtext>F<\/mml:mtext><\/mml:mrow><mml:mrow><mml:mtext>5a<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math> combined with cell-membrane lipids extracted from test MRSA or with 1,2-dipalmitoyl-<jats:italic>sn<\/jats:italic>-glycero-3-phospho-glycerol (DPPG) for possible molecular targets lying in MRSA cell-membrane. The results indicated that the anti-MRSA activity of azalomycin <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\"><mml:mrow><mml:msub><mml:mrow><mml:mtext>F<\/mml:mtext><\/mml:mrow><mml:mrow><mml:mtext>5a<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math> remarkably decreased when it combined with membrane lipids or DPPG. This indicated that cell-membrane lipids especially DPPG might be important targets of azalomycin <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M7\"><mml:mrow><mml:msub><mml:mrow><mml:mtext>F<\/mml:mtext><\/mml:mrow><mml:mrow><mml:mtext>5a<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math> against MRSA.<\/jats:p>","DOI":"10.1155\/2018\/6942452","type":"journal-article","created":{"date-parts":[[2018,1,21]],"date-time":"2018-01-21T18:33:44Z","timestamp":1516559624000},"page":"1-5","source":"Crossref","is-referenced-by-count":1,"title":["Mechanism of Azalomycin F<sub>5a<\/sub> against Methicillin-Resistant<i> Staphylococcus aureus<\/i>"],"prefix":"10.1155","volume":"2018","author":[{"given":"Li","family":"Xu","sequence":"first","affiliation":[{"name":"College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, 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