{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:07:16Z","timestamp":1740103636704,"version":"3.37.3"},"reference-count":36,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,11,21]],"date-time":"2018-11-21T00:00:00Z","timestamp":1542758400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Infectious Diseases in Obstetrics and Gynecology"],"published-print":{"date-parts":[[2018,11,21]]},"abstract":"<jats:p><jats:italic>Objectives<\/jats:italic>. To identify the prevalence and the types of<jats:italic>Neisseria gonorrhoeae<\/jats:italic>(NG) resistance plasmids-mediated penicillin (PPNG) and tetracycline (TRNG), the ciprofloxacin resistance (CRNG), and related risk factors of each types of resistance.<jats:italic>Methods<\/jats:italic>. The beta-lactamase-producing plasmid types (Africa, Asia, and Toronto),<jats:italic>tetM<\/jats:italic>tetracycline resistance plasmid types (America and Dutch), and the determination of the Ser-91 mutation of GyrA were detected by specifics PCRs on 149 diagnosed NG positives samples followed by<jats:italic>Hinf1<\/jats:italic>digestion for<jats:italic>tetM<\/jats:italic>and<jats:italic>gyrA<\/jats:italic>mutation.<jats:italic>Results<\/jats:italic>. 135 (90.1%) samples showed a profile of molecular resistance to at least one antibiotic with predominance of ciprofloxacin resistance. In fact, 36 (24.2%) and 69 (46.3%) cases harbored PPNG and TRNG, respectively, and 116 (77.9%) cases showed the mutation Ser-91 of GyrA (CRNG). From a total of 36 PPNG isolates, the Toronto, Asian, and Toronto\/Asian types were detected in 13 (36.1%), 10 (27.8%), and 13 (36.1%) cases, respectively, whereas the African type was not detected. In addition, the American type of TRNG was detected in 92.8% (64\/69) of cases, while the Dutch type was detected in 7.2% (5\/69) of cases. The association of demographics and clinical variables with NG resistance to ciprofloxacin, penicillin, and tetracycline was studied and the risk factors have been determined.<jats:italic>Conclusion<\/jats:italic>. Resistance to penicillin, tetracycline, and ciprofloxacin among NG samples positives remained at high levels in Morocco as determined by molecular profile. So, the use of molecular tools for NG antimicrobial resistance detection can help in the management and spread limitation of this infection.<\/jats:p>","DOI":"10.1155\/2018\/7263849","type":"journal-article","created":{"date-parts":[[2018,11,21]],"date-time":"2018-11-21T23:31:42Z","timestamp":1542843102000},"page":"1-11","source":"Crossref","is-referenced-by-count":15,"title":["Molecular Antimicrobial Resistance of<i>Neisseria gonorrhoeae<\/i>in a Moroccan Area"],"prefix":"10.1155","volume":"2018","author":[{"given":"Safae","family":"Karim","sequence":"first","affiliation":[{"name":"Laboratoire de Microbiologie et de Biologie Mol\u00e9culaire, Facult\u00e9 de M\u00e9decine et de Pharmacie de F\u00e8s (FMPF), Universit\u00e9 Sidi Mohammed Ben Abdellah (USMBA), F\u00e8s, Morocco"},{"name":"Laboratoire de Biotechnologie, Facult\u00e9 des Sciences Dhar El Mehraz, USMBA, F\u00e8s, Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chahrazed","family":"Bouchikhi","sequence":"additional","affiliation":[{"name":"Service de Gyn\u00e9cologie CHU Hassan II de F\u00e8s, Morocco"},{"name":"Equipe Micro-Organismes, G\u00e9nomique et Facteurs Oncog\u00e8nes, Laboratoire de Pathologie humaine, biom\u00e9decine et Environnement, FMPF, USMBA, F\u00e8s, Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdelaziz","family":"Banani","sequence":"additional","affiliation":[{"name":"Service de Gyn\u00e9cologie CHU Hassan II de F\u00e8s, Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hinde","family":"El Fatemi","sequence":"additional","affiliation":[{"name":"Equipe Anatomo-Pathologie, Laboratoire Central d\u2019Analyse M\u00e9dicale, CHU Hassan II, F\u00e8s, Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiatou","family":"Souho","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie et de Biologie Mol\u00e9culaire, Facult\u00e9 de M\u00e9decine et de Pharmacie de F\u00e8s (FMPF), Universit\u00e9 Sidi Mohammed Ben Abdellah (USMBA), F\u00e8s, Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanaa","family":"Erraghay","sequence":"additional","affiliation":[{"name":"Service de Gyn\u00e9cologie CHU Hassan II de F\u00e8s, Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5509-3991","authenticated-orcid":true,"given":"Bahia","family":"Bennani","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie et de Biologie Mol\u00e9culaire, Facult\u00e9 de M\u00e9decine et de Pharmacie de F\u00e8s (FMPF), Universit\u00e9 Sidi Mohammed Ben Abdellah (USMBA), F\u00e8s, Morocco"},{"name":"Equipe Micro-Organismes, G\u00e9nomique et Facteurs Oncog\u00e8nes, Laboratoire de Pathologie humaine, biom\u00e9decine et Environnement, FMPF, USMBA, F\u00e8s, Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0143304"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-14663-3_2"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000025"},{"issue":"1","key":"4","volume":"13","year":"2013","journal-title":"BMC Infectious Diseases"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1006\/plas.1999.1431"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e3181fc695a"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/32.5.667"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-016-1435-0"},{"issue":"2","key":"9","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1128\/JCM.38.2.521-525.2000","volume":"38","year":"2000","journal-title":"Journal of Clinical Microbiology"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2013-051166"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2009.03.009"},{"journal-title":"The Journal of Microbiology","first-page":"45","year":"2007","key":"11"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1586\/eri.09.63"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04964.x"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.00010-14"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-8080(12)40660-7"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(76)91918-8"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/155.4.819"},{"key":"19","first-page":"32","volume-title":"Phenotypic and molecular characterization of Neisseria gonorrhoeae isolated in Rio de Janeiro, Brazil, 2002-2003","volume":"16","year":"2004"},{"key":"20","first-page":"175","volume":"19","year":"2007","journal-title":"DST - 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