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Vasorelaxation of aortic rings in response to acetylcholine (AChIR) and to reduced pO<jats:sub>2<\/jats:sub> (HIR) was tested in vitro in the absence\/presence of NOS inhibitor L\u2010NAME and superoxide scavenger TEMPOL. eNOS, iNOS, antioxidative enzyme, and NADPH oxidase mRNA expression was assessed by qPCR. Serum oxidative stress markers and enzyme activity were assessed by spectrometry, and superoxide production was determined by DHE fluorescence. Impaired AChIR and HIR in the A\u2010HBO<jats:sub>2<\/jats:sub> group were restored by TEMPOL. L\u2010NAME inhibited AChIR in all groups. Serum oxidative stress and superoxide production were increased in the A\u2010HBO<jats:sub>2<\/jats:sub> group compared to all other groups. The mRNA expression of iNOS was decreased in the A\u2010HBO<jats:sub>2<\/jats:sub> and 24H\u2010HBO<jats:sub>2<\/jats:sub> groups while SOD1 and 3 and NADPH oxidase were increased in the 4D\u2010HBO<jats:sub>2<\/jats:sub> group. The expression and activity of catalase and glutathione peroxidase were increased in the 4D\u2010HBO<jats:sub>2<\/jats:sub> group as well. AChIR was NO dependent. Acute HBO<jats:sub>2<\/jats:sub> transiently impaired vasorelaxation due to increased oxidative stress. Vasorelaxation was restored and oxidative stress was normalized 24\u2009h after the treatment.<\/jats:p>","DOI":"10.1155\/2018\/7406027","type":"journal-article","created":{"date-parts":[[2018,4,29]],"date-time":"2018-04-29T23:31:41Z","timestamp":1525044701000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Acute Hyperbaric Oxygenation, Contrary to Intermittent Hyperbaric Oxygenation, Adversely Affects Vasorelaxation in Healthy Sprague\u2010Dawley Rats due to Increased Oxidative 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