{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,7]],"date-time":"2026-11-07T04:46:10Z","timestamp":1794026770039,"version":"4.8.0"},"reference-count":27,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,10,10]],"date-time":"2018-10-10T00:00:00Z","timestamp":1539129600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Microbiology"],"published-print":{"date-parts":[[2018,10,10]]},"abstract":"<jats:p><jats:italic>Objective<\/jats:italic>. The study aims to speciate clinical <jats:italic>Candida<\/jats:italic> isolates and detect their biofilm-forming ability and antifungal resistance. <jats:italic>Methods<\/jats:italic>. All the <jats:italic>Candida<\/jats:italic> spp. isolated from different clinical samples like pus, urine, blood, and body fluid were included in the study. Biofilm production was tested by the microtiter plate method. Antifungal susceptibility was studied by the disk diffusion method. Patient\u2019s demographic details such as age, sex, and clinical information were collected. Presence of other risk factors such as diabetes mellitus, history of antibiotic use, and any urinary tract instrumentations was also recorded. <jats:italic>Results<\/jats:italic>. Among 90 <jats:italic>Candida<\/jats:italic> species isolated, most predominant species was found to be <jats:italic>C. albicans<\/jats:italic> (45.5%) followed by <jats:italic>C. tropicalis<\/jats:italic> (28.88%), <jats:italic>C. krusei<\/jats:italic> (20%), <jats:italic>C. glabrata<\/jats:italic> (3.33%), and <jats:italic>C. parapsilosis<\/jats:italic> (2.22%). <jats:italic>Candida<\/jats:italic> spp. were isolated from urine (43%), BAL\/sputum (18.88%), high vaginal swab (8.88%), suction tips (7.77%), blood and wound swabs (6.66%), pus (3.33%), bile aspirate (2.22%), and deep tissue (1.11%). A larger number of females were affected than males, and the age group of 51 to 60 years was more susceptible to candidiasis. A higher number of <jats:italic>C. albicans<\/jats:italic> isolates produced biofilm followed by <jats:italic>C. parapsilosis<\/jats:italic>, <jats:italic>C. tropicalis<\/jats:italic>, and <jats:italic>C. krusei.<\/jats:italic> However, <jats:italic>C. glabrata<\/jats:italic> showed no biofilm production in our study. All <jats:italic>Candida<\/jats:italic> isolates were 100% sensitive to amphotericin B. Voriconazole was the next effective drug with 81.11% susceptibility. 24.44% of strains were resistant to fluconazole. <jats:italic>Conclusion<\/jats:italic>. Speciation of <jats:italic>Candida<\/jats:italic> isolates, detection of ability to form the biofilm, and monitoring of antifungal susceptibility testing are necessary for appropriate treatment.<\/jats:p>","DOI":"10.1155\/2018\/7495218","type":"journal-article","created":{"date-parts":[[2018,10,10]],"date-time":"2018-10-10T19:36:47Z","timestamp":1539200207000},"page":"1-5","source":"Crossref","is-referenced-by-count":91,"title":["Antifungal Susceptibility and Biofilm Production of <i>Candida<\/i> spp. Isolated from Clinical Samples"],"prefix":"10.1155","volume":"2018","author":[{"given":"Munmun B.","family":"Marak","sequence":"first","affiliation":[{"name":"Department of Microbiology, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5370-0663","authenticated-orcid":true,"given":"Biranthabail","family":"Dhanashree","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.4103\/0976-9668.82317"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00316-09"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00726-15"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.MB-0020-2015"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/456878"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.039354-0"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2009.04.018"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2011.07.004"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.4103\/0255-0857.124300"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.4103\/0255-0857.81794"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.4103\/0377-4929.142680"},{"key":"12","year":"2009"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.3906\/sag-1204-119"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.4103\/0974-777X.77288"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.9790\/0853-13524449"},{"key":"16","first-page":"198","volume":"4","year":"2012","journal-title":"Iran Journal of Microbiology"},{"key":"17","first-page":"171","volume":"104","year":"1996","journal-title":"Indian Journal of Microbiology Research"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.2147\/RRTM.S82984"},{"key":"19","first-page":"122","volume":"69","year":"2003","journal-title":"Indian Journal of Dermatology, Venereology, and Leprology"},{"key":"20","first-page":"157","volume":"3","year":"2011","journal-title":"International Journal of Pharmacy and Pharmaceutical Sciences"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.3201\/eid1001.030119"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1080\/13693780500338084"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1128\/iai.70.2.878-888.2002"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.21276\/IJRDPL"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.5812\/jjm.28057"},{"key":"26","first-page":"3193","volume":"2","year":"2014","journal-title":"School Journal of Applied Medical Science"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-51-6-479"}],"container-title":["International Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijmicro\/2018\/7495218.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijmicro\/2018\/7495218.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijmicro\/2018\/7495218.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,10]],"date-time":"2018-10-10T19:36:49Z","timestamp":1539200209000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/ijmicro\/2018\/7495218\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,10]]},"references-count":27,"alternative-id":["7495218","7495218"],"URL":"https:\/\/doi.org\/10.1155\/2018\/7495218","relation":{},"ISSN":["1687-918X","1687-9198"],"issn-type":[{"value":"1687-918X","type":"print"},{"value":"1687-9198","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,10]]}}}