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The detection effect of the <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mrow><mml:msub><mml:mrow><mml:mi>T<\/mml:mi><\/mml:mrow><mml:mrow><mml:mi mathvariant=\"normal\">m<\/mml:mi><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>-shift was assessed through the stability, sensitivity, accuracy test, and clinical detection. The results showed that these three sets of primers could distinguish accurately between<jats:italic> A. ceylanicum<\/jats:italic> and<jats:italic> A. caninum<\/jats:italic>. The coefficient of variation in their <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mrow><mml:msub><mml:mrow><mml:mi>T<\/mml:mi><\/mml:mrow><mml:mrow><mml:mi mathvariant=\"normal\">m<\/mml:mi><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math> values on the three SNPs was 0.09% and 0.15% (ITS71), 0.18% and 0.14% (ITS197), and 0.13% and 0.07% (ITS296), respectively. The lowest detectable concentration of standard plasmids for<jats:italic> A. ceylanicum<\/jats:italic> and<jats:italic> A. caninum<\/jats:italic> was 5.33 \u00d7 10<jats:sup>\u22126<\/jats:sup>\u2009ng\/<jats:italic>\u03bc<\/jats:italic>L and 5.03 \u00d7 10<jats:sup>\u22126<\/jats:sup>\u2009ng\/<jats:italic>\u03bc<\/jats:italic>L. The <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mrow><mml:msub><mml:mrow><mml:mi>T<\/mml:mi><\/mml:mrow><mml:mrow><mml:mi mathvariant=\"normal\">m<\/mml:mi><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>-shift results of ten DNA samples from the dog-derived hookworms were consistent with their known species. In the clinical detection of 50 fecal samples from stray dogs, the positive rate of hookworm detected by <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\"><mml:mrow><mml:msub><mml:mrow><mml:mi>T<\/mml:mi><\/mml:mrow><mml:mrow><mml:mi mathvariant=\"normal\">m<\/mml:mi><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>-shift (42%) was significantly higher than that by microscopic examination (34%), and the former can identify the<jats:italic> Ancylostoma<\/jats:italic> species. It is concluded that the <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M7\"><mml:mrow><mml:msub><mml:mrow><mml:mi>T<\/mml:mi><\/mml:mrow><mml:mrow><mml:mi mathvariant=\"normal\">m<\/mml:mi><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>-shift method is rapid, specific, sensitive, and suitable for the clinical detection and zoonotic risk assessment of the dog-derived hookworm.<\/jats:p>","DOI":"10.1155\/2018\/7617094","type":"journal-article","created":{"date-parts":[[2018,5,13]],"date-time":"2018-05-13T19:32:40Z","timestamp":1526239960000},"page":"1-8","source":"Crossref","is-referenced-by-count":3,"title":["<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:msub><mml:mrow><mml:mi>T<\/mml:mi><\/mml:mrow><mml:mrow><mml:mi mathvariant=\"normal\">m<\/mml:mi><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>-Shift Detection of Dog-Derived<i> Ancylostoma ceylanicum<\/i> and<i> A. 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