{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T01:12:36Z","timestamp":1758935556307,"version":"3.37.3"},"reference-count":30,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,6,11]],"date-time":"2018-06-11T00:00:00Z","timestamp":1528675200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"KRIBB Research Initiative Program","award":["ABC-2015M3A6A2074238"],"award-info":[{"award-number":["ABC-2015M3A6A2074238"]}]},{"DOI":"10.13039\/501100003621","name":"Ministry of Science, ICT and Future Planning","doi-asserted-by":"publisher","award":["ABC-2015M3A6A2074238"],"award-info":[{"award-number":["ABC-2015M3A6A2074238"]}],"id":[{"id":"10.13039\/501100003621","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2018,6,11]]},"abstract":"<jats:p>The study describes the development of a vaccine using microcrystalline cellulose (Avicel PH-101) as a delivery carrier of recombinant protein-based antigen against erysipelas. Recombinant SpaA, surface protective protein, from a gram-positive pathogen<jats:italic>Erysipelothrix rhusiopathiae<\/jats:italic>was fused to a cellulose-binding domain (CBD) from<jats:italic>Trichoderma harzianum<\/jats:italic>endoglucanase II through a S3N10 peptide. The fusion protein (CBD-SpaA) was expressed in<jats:italic>Escherichia coli<\/jats:italic>and was subsequently bound to Avicel PH-101. The antigenicity of CBD-SpaA bound to the Avicel was evaluated by enzyme-linked immunosorbent (ELISA) and confocal laser scanning microscope (CLSM) assays. For the examination of its immunogenicity, groups of mice were immunized with different constructs (soluble CBD-SpaA, Avicel coated with CBD-SpaA, whole bacterin of<jats:italic>E. rhusiopathiae<\/jats:italic>(positive control), and PBS (negative control)). In two weeks after immunization, mice were challenged with 1x10<jats:sup>7<\/jats:sup>CFU of<jats:italic>E. rhusiopathiae<\/jats:italic>and Avicel coated with CBD-SpaA induced protective immunity in mice. In conclusion, this study demonstrates the feasibility of microcrystalline cellulose as the delivery system of recombinant protein subunit vaccine against<jats:italic>E. rhusiopathiae<\/jats:italic>infection in mice.<\/jats:p>","DOI":"10.1155\/2018\/7670505","type":"journal-article","created":{"date-parts":[[2018,6,11]],"date-time":"2018-06-11T23:34:44Z","timestamp":1528760084000},"page":"1-7","source":"Crossref","is-referenced-by-count":7,"title":["Microcrystalline Cellulose for Delivery of Recombinant Protein-Based Antigen against Erysipelas in Mice"],"prefix":"10.1155","volume":"2018","author":[{"given":"Wooyoung","family":"Jeon","sequence":"first","affiliation":[{"name":"Biotechnology Process Engineering Center, KRIBB, Cheongju 363-883, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeu-Chun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 305-701, Republic of 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