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Alkylating agents (AAs) are able to induce alkylation in macromolecules, causing DNA damage, as DNA methylation. Our objective was to evaluate atorvastatin (AVA) antimutagenic, cytoprotective, and antigenotoxic potentials against DNA lesions caused by AA. AVA chemopreventive ability was evaluated using antimutagenicity assays (<jats:italic>Salmonella<\/jats:italic>\/microsome assay), cytotoxicity, cell cycle, and genotoxicity assays in HepG2 cells. The cells were cotreated with AVA and the AA methyl methanesulfonate (MMS) or cyclophosphamide (CPA). Our datum showed that AVA reduces the alkylation\u2010mediated DNA damage in different <jats:italic>in vitro<\/jats:italic> experimental models. Cytoprotection of AVA at low doses (0.1\u20131.0\u2009<jats:italic>\u03bc<\/jats:italic>M) was observed after 24\u2009h of cotreatment with MMS or CPA at their LC<jats:sub>50<\/jats:sub>, causing an increase in HepG2 survival rates. After all, AVA at 10\u2009<jats:italic>\u03bc<\/jats:italic>M and 25\u2009<jats:italic>\u03bc<\/jats:italic>M had decreased effect in micronucleus formation in HepG2 cells and restored cell cycle alterations induced by MMS and CPA. This study supports the hypothesis that statins can be chemopreventive agents, acting as antimutagenic, antigenotoxic, and cytoprotective components, specifically against alkylating agents of DNA.<\/jats:p>","DOI":"10.1155\/2018\/7820890","type":"journal-article","created":{"date-parts":[[2018,4,3]],"date-time":"2018-04-03T23:32:09Z","timestamp":1522798329000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Atorvastatin Downregulates <i>In Vitro<\/i> Methyl Methanesulfonate and Cyclophosphamide Alkylation\u2010Mediated Cellular and DNA Injuries"],"prefix":"10.1155","volume":"2018","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9278-4441","authenticated-orcid":false,"given":"Carlos F.","family":"Araujo-Lima","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa S. 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