{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:19:08Z","timestamp":1787167148966,"version":"build-2736575974"},"reference-count":19,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,9,18]],"date-time":"2018-09-18T00:00:00Z","timestamp":1537228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hassan II University Hospital"},{"name":"Sidi Mohamed ben Abdellah University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Disease Markers"],"published-print":{"date-parts":[[2018,9,18]]},"abstract":"<jats:p>\n                    Ewing sarcoma\/primitive neuroectodermal tumor (Ewing\/PNET sarcomas or EPS) are a group of round cell tumors. Malignant round cell tumors form a large and diverse group that includes rhabdomyosarcoma, synovial sarcoma, non-Hodgkin\u2019s lymphoma, neuroblastoma, hepatoblastoma, Wilm\u2019s tumor, desmoplastic small round cell tumor, and other morphologically similar entities. Differential diagnosis of Ewing sarcoma\/primitive neuroectodermal tumor (Ewing\/PNET sarcomas or EPS) is difficult. In addition to morphology and immunohistochemistry (IHC), differential diagnosis of these tumors is based on molecular analysis of the\n                    <jats:italic>EWSR1<\/jats:italic>\n                    gene rearrangement using fluorescent in situ hybridization (FISH) technique. We investigated the diagnostic value of combined CD99 immunostaining and\n                    <jats:italic>EWSR1<\/jats:italic>\n                    t(22q12) alteration using a dual-color, break-apart rearrangement probe in forty-one formalin-fixed paraffin-embedded (FFPE) tissue samples from pediatric and adult patients diagnosed with EPS. IHC was performed in all cases using the CD99 antibody and showed a positivity of 92.7% in the enrolled cases (38\/41) followed by FISH analysis where 48.8% of the cases (20\/41) were rearranged. Sensitivity and specificity for IHC assays were 88% and 58%, respectively. Notably, FISH had a sensitivity of 100% and a specificity of 87%. In addition, CD99 positivity was found to correlate with\n                    <jats:italic>EWSR1<\/jats:italic>\n                    rearrangement (\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                      <mml:mi>p<\/mml:mi>\n                      <mml:mo>&lt;<\/mml:mo>\n                      <mml:mn>0.05<\/mml:mn>\n                    <\/mml:math>\n                    ). This report shows that FISH has better sensitivity and specificity than IHC in the Moroccan population, and supports its combination with CD99 immunostaining as diagnostic biomarkers for this rare malignant entity.\u201d\n                  <\/jats:p>","DOI":"10.1155\/2018\/7971019","type":"journal-article","created":{"date-parts":[[2018,9,18]],"date-time":"2018-09-18T19:31:17Z","timestamp":1537299077000},"page":"1-5","source":"Crossref","is-referenced-by-count":10,"title":["<i>EWSR1<\/i>\n                    Rearrangement and CD99 Expression as Diagnostic Biomarkers for Ewing\/PNET Sarcomas in a Moroccan Population"],"prefix":"10.1155","volume":"2018","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6937-7285","authenticated-orcid":true,"given":"Sara","family":"Louati","sequence":"first","affiliation":[{"name":"Bioactive Molecules, Structure and Functions Laboratory, Faculty of Sciences and Techniques, Sidi Mohamed ben Abdellah University of Fez, Morocco"},{"name":"Pathological Anatomy and Molecular Pathology Department, Hassan II University Hospital of Fez, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"Senhaji","sequence":"additional","affiliation":[{"name":"Bioactive Molecules, Structure and Functions Laboratory, Faculty of Sciences and Techniques, Sidi Mohamed ben Abdellah University of Fez, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laila","family":"Chbani","sequence":"additional","affiliation":[{"name":"Pathological Anatomy and Molecular Pathology Department, Hassan II University Hospital of Fez, Morocco"},{"name":"Biomedical and Translational Research Laboratory, Faculty of Medicine and Pharmacy, Sidi Mohamed ben Abdellah University of Fez, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanae","family":"Bennis","sequence":"additional","affiliation":[{"name":"Pathological Anatomy and Molecular Pathology Department, Hassan II University Hospital of Fez, Morocco"},{"name":"Biomedical and Translational Research Laboratory, Faculty of Medicine and Pharmacy, Sidi Mohamed ben Abdellah University of Fez, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","first-page":"17","volume":"21","year":"1921","journal-title":"Proceedings of the New York Pathological Society"},{"key":"2","year":"2013"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.annpat.2014.11.001"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1053\/j.semdp.2014.01.002"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-009-0828-5"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1007\/s12032-012-0412-8"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.4103\/0019-5413.69304"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.2350\/11-08-1078-PB.1"},{"issue":"4","key":"9","first-page":"203","volume":"19","year":"2009","journal-title":"EJIFCC"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1111\/his.12269"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2016.05.008"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2005.02158.x"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800503"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2006.02535.x"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.7754\/clin.lab.2013.130909"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(02)00680-5"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2017.4"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdu254"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.3109\/08880018.2011.622034"}],"container-title":["Disease Markers"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/dm\/2018\/7971019.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/dm\/2018\/7971019.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/dm\/2018\/7971019.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,9,18]],"date-time":"2018-09-18T19:31:20Z","timestamp":1537299080000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/dm\/2018\/7971019\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,9,18]]},"references-count":19,"alternative-id":["7971019","7971019"],"URL":"https:\/\/doi.org\/10.1155\/2018\/7971019","relation":{},"ISSN":["0278-0240","1875-8630"],"issn-type":[{"value":"0278-0240","type":"print"},{"value":"1875-8630","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,9,18]]}}}