{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T03:14:31Z","timestamp":1777518871009,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,10,31]],"date-time":"2018-10-31T00:00:00Z","timestamp":1540944000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Tropical Medicine"],"published-print":{"date-parts":[[2018,10,31]]},"abstract":"<jats:p><jats:italic>Helicobacter pylori<\/jats:italic> (<jats:italic>H. pylori<\/jats:italic>) is a gram-negative bacterium that colonizes the human stomach. Infection with this microaerophilic bacterium causes gastric and duodenal ulcer. This study sought to isolate<jats:italic> H. pylori<\/jats:italic>, from gastric biopsy samples of dyspeptic patients in Ghana using a 2,3,5-triphenyltetrazolium chloride (TTC) dye incorporated medium method. This TTC dye method was further used in an antimicrobial susceptibility assay involving<jats:italic> Dissotis rotundifolia<\/jats:italic> extract (DRE).<jats:italic> H. pylori<\/jats:italic> were successfully isolated from gastric biopsy of dyspeptic patients. Pure cultures of<jats:italic> H. pylori<\/jats:italic> in 2,3,5-triphenyltetrazolium chloride (TTC) dye incorporated medium were seen as sparkling colonies. Isolates, identified as<jats:italic> H. pylori<\/jats:italic>, were gram-negative and urease, catalase, and oxidase positive and showed characteristic morphology as spiral-shaped bacteria under the microscope. The organisms were found to be susceptible to cephalothin and resistant to nalidixic acid. Above all, the observation that<jats:italic> H. pylori<\/jats:italic> grew only at 37\u00b0C and not 25\u00b0C or 42\u00b0C affirms that the bacterium is neither<jats:italic> Helicobacter cinaedi<\/jats:italic> nor<jats:italic> Helicobacter fenneliae.<\/jats:italic> The anti-<jats:italic>H. pylori<\/jats:italic> study depicts a statistically lower zone of inhibition for DRE compared to standard drugs [amoxicillin and clarithromycin] (p&lt;0.05), whereas metronidazole showed no zone of inhibition. This study reports the first successful isolation and culturing of<jats:italic> H. pylori<\/jats:italic> in Ghana using TTC dye. It also shows that DRE possess an<jats:italic> in vitro<\/jats:italic> anti-<jats:italic>H. pylori<\/jats:italic> activity and that DRE has some therapeutic potential against<jats:italic> H. pylori<\/jats:italic> infection.<\/jats:p>","DOI":"10.1155\/2018\/8071081","type":"journal-article","created":{"date-parts":[[2018,10,31]],"date-time":"2018-10-31T19:31:42Z","timestamp":1541014302000},"page":"1-6","source":"Crossref","is-referenced-by-count":8,"title":["Isolation of<i> Helicobacter pylori<\/i> from Gastric Biopsy of Dyspeptic Patients in Ghana and<i> In Vitro<\/i> Preliminary Assessment of the Effect of<i> Dissotis rotundifolia<\/i> Extract on Its Growth"],"prefix":"10.1155","volume":"2018","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8287-7460","authenticated-orcid":true,"given":"Michael Buenor","family":"Adinortey","sequence":"first","affiliation":[{"name":"Department of Biochemistry, School of Biological Sciences, College of Agriculture and Natural Sciences, University of 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Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin Tangnaa","family":"Morna","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, Cape Coast, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Damian H.","family":"Amewowor","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biotechnology, School of Biological Sciences, College of Agriculture and Natural Sciences, University of Cape Coast, Cape Coast, Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(83)92719-8"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.11.032"},{"issue":"1","key":"3","first-page":"80","volume":"21","year":"2012","journal-title":"Mymensingh Medical 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