{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T22:46:11Z","timestamp":1772750771851,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,11,29]],"date-time":"2018-11-29T00:00:00Z","timestamp":1543449600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"UK Ministry of Defence"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2018,11,29]]},"abstract":"<jats:p>There is a requirement for an efficacious vaccine to protect people against infection from<jats:italic>Francisella tularensis<\/jats:italic>, the etiological agent of tularemia. The lipopolysaccharide (LPS) of<jats:italic>F. tularensis<\/jats:italic>is suboptimally protective against a parenteral lethal challenge in mice. To develop a more efficacious subunit vaccine, we have used a novel biosynthetic technique of protein glycan coupling technology (PGCT) that exploits bacterial N-linked glycosylation to recombinantly conjugate<jats:italic>F. tularensis<\/jats:italic>O-antigen glycans to the immunogenic carrier protein<jats:italic>Pseudomonas aeruginosa<\/jats:italic>exoprotein A (ExoA). Previously, we demonstrated that an ExoA glycoconjugate with two glycosylation sequons was capable of providing significant protection to mice against a challenge with a low-virulence strain of<jats:italic>F. tularensis<\/jats:italic>. Here, we have generated a more heavily glycosylated conjugate vaccine and evaluated its efficacy in a Fischer 344 rat model of tularemia. We demonstrate that this glycoconjugate vaccine protected rats against disease and the lethality of an inhalational challenge with<jats:italic>F. tularensis<\/jats:italic>Schu S4. Our data highlights the potential of this biosynthetic approach for the creation of next-generation tularemia subunit vaccines.<\/jats:p>","DOI":"10.1155\/2018\/8087916","type":"journal-article","created":{"date-parts":[[2018,11,29]],"date-time":"2018-11-29T23:31:04Z","timestamp":1543534264000},"page":"1-12","source":"Crossref","is-referenced-by-count":29,"title":["An O-Antigen Glycoconjugate Vaccine Produced Using Protein Glycan Coupling Technology Is Protective in an Inhalational Rat Model of Tularemia"],"prefix":"10.1155","volume":"2018","author":[{"given":"Laura E.","family":"Marshall","sequence":"first","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle","family":"Nelson","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, 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