{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T02:16:23Z","timestamp":1790734583210,"version":"4.1.0"},"reference-count":57,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,10,22]],"date-time":"2018-10-22T00:00:00Z","timestamp":1540166400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"French Minister of Research"},{"name":"CNRS UMR 7267 Laboratory"},{"name":"State-Region Planning Contracts (CPER)"},{"name":"European Regional Development Fund (FEDER)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2018,10,22]]},"abstract":"<jats:p><jats:italic>Legionella pneumophila<\/jats:italic>, the causative agent of Legionnaires\u2019 disease, is a waterborne bacterium mainly found in man-made water systems in close association with free-living amoebae and multispecies biofilms.<jats:italic> Pseudomonas<\/jats:italic> strains, originating from various environments including freshwater systems or isolated from hospitalized patients, were tested for their antagonistic activity towards<jats:italic> L. pneumophila<\/jats:italic>. A high amount of tested strains was thus found to be active. This antibacterial activity was correlated to the presence of tensioactive agents in culture supernatants. As<jats:italic> Pseudomonas<\/jats:italic> strains were known to produce biosurfactants, these compounds were specifically extracted and purified from active strains and further characterized using reverse-phase HPLC and mass spectrometry methods. Finally, all biosurfactants tested (lipopeptides and rhamnolipids) were found active and this activity was shown to be higher towards<jats:italic> Legionella <\/jats:italic>strains compared to various other bacteria. Therefore, described biosurfactants are potent anti-<jats:italic>Legionella <\/jats:italic>agents that could be used in the water treatment industry although tests are needed to evaluate how effective they would be under field conditions.<\/jats:p>","DOI":"10.1155\/2018\/8194368","type":"journal-article","created":{"date-parts":[[2018,10,22]],"date-time":"2018-10-22T19:41:52Z","timestamp":1540237312000},"page":"1-15","source":"Crossref","is-referenced-by-count":25,"title":["Highlighting the Potency of Biosurfactants Produced by<i> Pseudomonas<\/i> Strains as Anti-<i>Legionella<\/i> Agents"],"prefix":"10.1155","volume":"2018","author":[{"given":"Cl\u00e9mence","family":"Loiseau","sequence":"first","affiliation":[{"name":"Laboratoire Ecologie & Biologie des Interactions, UMR CNRS 7267, Universit\u00e9 de Poitiers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilie","family":"Portier","sequence":"additional","affiliation":[{"name":"Laboratoire Ecologie & 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