{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T14:35:41Z","timestamp":1787668541445,"version":"build-2736575974"},"reference-count":50,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,12,16]],"date-time":"2018-12-16T00:00:00Z","timestamp":1544918400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST 106-2320-B-007-001"],"award-info":[{"award-number":["MOST 106-2320-B-007-001"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["DOH100-TD-C-111-008"],"award-info":[{"award-number":["DOH100-TD-C-111-008"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["NSC94-2314-B-1282-064"],"award-info":[{"award-number":["NSC94-2314-B-1282-064"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Department of Health, Taiwan","award":["MOST 106-2320-B-007-001"],"award-info":[{"award-number":["MOST 106-2320-B-007-001"]}]},{"name":"Department of Health, Taiwan","award":["DOH100-TD-C-111-008"],"award-info":[{"award-number":["DOH100-TD-C-111-008"]}]},{"name":"Department of Health, Taiwan","award":["NSC94-2314-B-1282-064"],"award-info":[{"award-number":["NSC94-2314-B-1282-064"]}]},{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"publisher","award":["MOST 106-2320-B-007-001"],"award-info":[{"award-number":["MOST 106-2320-B-007-001"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"publisher","award":["DOH100-TD-C-111-008"],"award-info":[{"award-number":["DOH100-TD-C-111-008"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"publisher","award":["NSC94-2314-B-1282-064"],"award-info":[{"award-number":["NSC94-2314-B-1282-064"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2018,12,16]]},"abstract":"<jats:p>Limited information is currently available concerning HLA class I antigen abnormalities in sarcomatoid hepatocellular carcinoma (sHCC). Here, we have analyzed the growth characteristics and HLA class I antigen status of four sHCC cell lines (sHCC29, sHCC63, sHCC74, and SAR-HCV); the first three were newly established in this study. Among the four, sHCC29 showed the highest growth rate<jats:italic>in vitro<\/jats:italic>and tumorigenicity in NOD-SCID mice. Unlike sHCC74 and SAR-HCV, both sHCC29 and sHCC63 had no detectable surface HLA class I antigen expression, alongside undetected intracellular<jats:italic>\u03b2<\/jats:italic><jats:sub>2<\/jats:sub>-microglobulin (<jats:italic>\u03b2<\/jats:italic><jats:sub>2<\/jats:sub>m) and marked HLA class I heavy chain and selective antigen-processing machinery (APM) component downregulation. The loss of<jats:italic>\u03b2<\/jats:italic><jats:sub>2<\/jats:sub>m in sHCC29 and sHCC63 was caused by a &gt;49\u2009kb deletion across the<jats:italic>B2M<\/jats:italic>locus, while their downregulation of APM components was transcriptional, reversible by IFN-<jats:italic>\u03b3<\/jats:italic>only in several components.<jats:italic>\u03b2<\/jats:italic><jats:sub>2<\/jats:sub>m was also undetected in the primary HCC lesions of the patients involved, indicating its<jats:italic>in vivo<\/jats:italic>relevance. We report for the first time HLA class I antigen loss with underlying<jats:italic>B2M<\/jats:italic>gene deficiency and APM defects in 50% (2 of 4) of the sHCC cell lines tested. These findings may have implications for a proper design of T cell immunotherapy for the treatment of sHCC patients.<\/jats:p>","DOI":"10.1155\/2018\/8363265","type":"journal-article","created":{"date-parts":[[2018,12,16]],"date-time":"2018-12-16T23:31:36Z","timestamp":1545003096000},"page":"1-12","source":"Crossref","is-referenced-by-count":6,"title":["Total HLA Class I Antigen Loss with the Downregulation of Antigen-Processing Machinery Components in Two Newly Established Sarcomatoid Hepatocellular Carcinoma Cell Lines"],"prefix":"10.1155","volume":"2018","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0396-8677","authenticated-orcid":true,"given":"Wei-Yi","family":"Lei","sequence":"first","affiliation":[{"name":"Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan"},{"name":"College of Medicine, Tzu Chi University, Hualien, Taiwan"},{"name":"Department of Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shih-Chieh","family":"Hsiung","sequence":"additional","affiliation":[{"name":"Division of Urology, Department of Surgery, Chang Gung Memorial Hospital, Taoyuan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao-Hsuan","family":"Wen","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chin-Hsuan","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Division of Urology, Department of Surgery, Chang Gung Memorial Hospital, Taoyuan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9084-8210","authenticated-orcid":true,"given":"Chien-Lin","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan"},{"name":"College of Medicine, Tzu Chi University, Hualien, Taiwan"},{"name":"Department of Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1897-9520","authenticated-orcid":true,"given":"Christopher Glenn","family":"Wallace","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Taoyuan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7537-3133","authenticated-orcid":true,"given":"Chien-Chung","family":"Chang","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu, Taiwan"},{"name":"Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1727-1280","authenticated-orcid":true,"given":"Shuen-Kuei","family":"Liao","sequence":"additional","affiliation":[{"name":"The Ph.D. Program for Cancer Biology and Drug Discovery, Taipei Medical University, Taipei City, Taiwan"},{"name":"Vectorite Biomedica\/U-Well Biopharma Inc., New Taipei City, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1746.2002.02747.x"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.3322\/canjclin.55.2.74"},{"key":"3","first-page":"191","volume-title":"Mesenchymal tumors of the liver","year":"2000"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1038\/nrc822"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0004355"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2013.01.005"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1007\/BF00647229"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1746.2003.02888.x"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinimag.2006.07.004"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1097\/01.pas.0000213431.07116.e0"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-014-3779-2"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(95)80033-6"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1038\/ni1102-999"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.10290"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000094807.95420.fe"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.1440"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1038\/nri1919"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1305133"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1177\/107327481402100112"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djt184"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1994.tb06089.x"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v8.i4.654"},{"issue":"1","key":"23","first-page":"129","volume":"31","year":"2011","journal-title":"Anticancer Research"},{"key":"24","first-page":"150","volume":"41","year":"2000","journal-title":"Proceedings of the American Association for Cancer Research"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(78)90296-9"},{"issue":"3","key":"26","first-page":"457","volume":"52","year":"1984","journal-title":"Immunology"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.6.3275"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1983.tb01176.x"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.4.1918"},{"issue":"7","key":"30","doi-asserted-by":"crossref","first-page":"2299","DOI":"10.4049\/jimmunol.137.7.2299","volume":"137","year":"1986","journal-title":"The Journal of Immunology"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-5890(98)00026-1"},{"issue":"5","key":"32","doi-asserted-by":"crossref","first-page":"2471","DOI":"10.4049\/jimmunol.130.5.2471","volume":"130","year":"1983","journal-title":"The Journal of Immunology"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2005.00462.x"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2005.02.006"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(03)00224-2"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2011.91"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0530291100"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.14777"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-012-1321-6"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114984"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.3.1462"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.4.1459"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/708036"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2013.10.105"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.676130"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1186\/s40425-016-0127-z"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.1038\/35101072"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1186\/s40425-016-0124-2"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.5280"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-012-1254-0"}],"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2018\/8363265.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2018\/8363265.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2018\/8363265.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T06:13:49Z","timestamp":1694499229000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/jir\/2018\/8363265\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,16]]},"references-count":50,"alternative-id":["8363265","8363265"],"URL":"https:\/\/doi.org\/10.1155\/2018\/8363265","relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"value":"2314-8861","type":"print"},{"value":"2314-7156","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12,16]]}}}