{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T18:08:04Z","timestamp":1782151684319,"version":"3.54.5"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,4,23]],"date-time":"2018-04-23T00:00:00Z","timestamp":1524441600000},"content-version":"vor","delay-in-days":112,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:p><jats:italic>Glycyrrhiza glabra<\/jats:italic> L. (Leguminosae) is widely used in folk medicines. Glycyrrhizin, an active compound of <jats:italic>G. glabra<\/jats:italic>, possesses anti\u2010inflammatory activity. This study investigates the <jats:italic>G. glabra<\/jats:italic> methanol extract and glycyrrhizin for the treatment of corneal neovascularization (CNV). <jats:italic>G. glabra<\/jats:italic> was extracted in 70% aqueous methanol. Phytochemical tests, thin layer chromatography (TLC), and high performance liquid chromatography (HPLC) were used for the analysis of chemical composition. The topical solution of <jats:italic>G. glabra<\/jats:italic> methanol extract (2%\u2009w\/v) and glycyrrhizin (1%\u2009w\/v) was prepared in normal saline. After corneal burn (1\u2009N NaOH), animals were left untreated for a week so that neovascularization appears in all groups. Treatments started on day 7 and continued for next 21 consecutive days. The animals were treated with 3 drops of various topical solutions thrice a day. Digital photograph analysis and histological studies were used for the evaluation of CNV. Phytochemical analysis of the <jats:italic>G. glabra<\/jats:italic> methanol extract showed the presence of saponins, phenols, carbohydrates, flavonoids, and proteins. TLC and HPLC confirmed the presence of glycyrrhizin. Photograph analysis of the extract and glycyrrhizin treated group showed a considerable decrease in CNV. Histological study of <jats:italic>G. glabra<\/jats:italic> and glycyrrhizin treated groups showed no blood vessels with properly arranged collagen fibers. This study showed that <jats:italic>G. glabra<\/jats:italic> and glycyrrhizin can be used for the treatment of CNV. Bioassay guided isolation can lead to preparation of ophthalmic solutions for the treatment of CNV.<\/jats:p>","DOI":"10.1155\/2018\/8438101","type":"journal-article","created":{"date-parts":[[2018,4,23]],"date-time":"2018-04-23T23:33:02Z","timestamp":1524526382000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Inhibitory Effects of <i>Glycyrrhiza glabra<\/i> and Its Major Constituent Glycyrrhizin on Inflammation\u2010Associated Corneal Neovascularization"],"prefix":"10.1155","volume":"2018","author":[{"given":"Syed Luqman","family":"Shah","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8133-6061","authenticated-orcid":false,"given":"Fazli","family":"Wahid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noorullah","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Umar","family":"Farooq","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6032-3915","authenticated-orcid":false,"given":"Abdul Jabbar","family":"Shah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shah","family":"Tareen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiaz","family":"Ahmad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9183-278X","authenticated-orcid":false,"given":"Taous","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,4,23]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2005.04.015"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2015.08.001"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2005.01.012"},{"key":"e_1_2_9_4_2","first-page":"118","article-title":"Antibacterial activity of Glycyrrhiza glabra against oral pathogens: an in vitro study","volume":"2","author":"Sedighinia F.","year":"2012","journal-title":"Avicenna Journal of Phytomedicine"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-8741(84)90017-5"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-8741(85)90078-9"},{"key":"e_1_2_9_7_2","first-page":"1","article-title":"Review on Glycyrrhiza glabra: licorice","volume":"1","author":"Kumar A.","year":"2012","journal-title":"Journal of Pharmaceutical and Scientific Innovation"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.3109\/13880208709088129"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2005.07.002"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2004.11.004"},{"key":"e_1_2_9_11_2","first-page":"379","volume-title":"Bioactive compounds from natural sources: isolation, characterization and biological properties","author":"Christensen S. 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