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The highly prevalent, chronic, and distinctive complexion changes on patients have imposed enormous psychic and economic burden on both individuals and society. Traditional Chinese Medicine (TCM) is a kind of precious source on chronic disease treatment, including skin dysfunctional diseases. In our previous study, a new compound named apigenin\u20107\u2010butylene glucoside has been authenticated and purified from a prescription of Chinese traditional medicine formula which has been used clinically in vitiligo treatment. The aim of this work is to evaluate the effects of this compound on melanogenesis using melanoma cell B16\u2010F10 <jats:italic>in vitro<\/jats:italic>. The results showed that apigenin\u20107\u2010butylene glucoside had almost no cytotoxicity on B16\u2010F10 cells within a lower dose of 5.0\u2009<jats:italic>\u03bc<\/jats:italic>g ml<jats:sup>\u22121<\/jats:sup> and enhanced the melanin level to about 41% and tyrosinase activity to 1.32\u2010fold when compared with controls. The compound showed minor cytotoxicity to B16\u2010F10 cells at the higher concentration of 10\u2009<jats:italic>\u03bc<\/jats:italic>g ml<jats:sup>\u22121<\/jats:sup> and 50\u2009<jats:italic>\u03bc<\/jats:italic>g ml<jats:sup>\u22121<\/jats:sup>, the inhibition rate was 8.4% and 11.8%, and the melanin level and tyrosinase activity showed a decreased trend because of the lower cell number at the higher concentrations. The results indicated that apigenin\u20107\u2010butylene glucoside was safe to B16\u2010F10 cells within a lower concentration, &lt;5.0\u2009<jats:italic>\u03bc<\/jats:italic>g ml<jats:sup>\u22121<\/jats:sup>. Incubated with 5.0\u2009ug ml<jats:sup>\u22121<\/jats:sup>of apigenin\u20107\u2010butylene glucoside for 48 hours, the mRNA and protein levels of <jats:italic>Tyr<\/jats:italic>, <jats:italic>Trp-1<\/jats:italic>, and <jats:italic>Trp-2<\/jats:italic> genes were all increased except <jats:italic>Mitf<\/jats:italic> in B16\u2010F10 cells. The stimulation of apigenin\u20107\u2010butylene glucoside on melanogenesis of B16\u2010F10 cells through Tyr, Trp\u20101, and Trp\u20102 pathway highlighted the potential usage of the compound in vitiligo treatment.<\/jats:p>","DOI":"10.1155\/2018\/8485670","type":"journal-article","created":{"date-parts":[[2018,11,18]],"date-time":"2018-11-18T23:39:28Z","timestamp":1542584368000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["A Newly Authenticated Compound from Traditional Chinese Medicine Decoction Induces Melanogenesis in B16\u2010F10 Cells by Increasing Tyrosinase Activity"],"prefix":"10.1155","volume":"2018","author":[{"given":"Xiu 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