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Different from cell study, this ex vivo study aims to investigate the biological effects of nanosecond pulsed electric field (nsPEF) on an independent multimembrane parasite, human hydatid cyst, to observe the unique influence of nanopulse on macromembrane structure, permeabilization, and biochemistry. The 300\u2009ns nsPEF was delivered on an experimental model of single human hydatid cyst ex vivo with eight different parameters. Then pathological changes during 7 days of 48 parasite cysts were followed up after nsPEF. The laminated layer, the germinal layer, the protoscolex, and cyst fluid were evaluated by the morphological, pathological, and biochemical measurements. The parameter screening found that nsPEF can damage hydatid cyst effectively when the field strength is higher than 14\u2009kV\/cm. When nsPEF is higher than 29\u2009kV\/cm, nsPEF destroy hydatid cyst completely by collapsing the germinal layer, destructing protoscolices, and exhausting the nutrition.<\/jats:p>","DOI":"10.1155\/2018\/8497283","type":"journal-article","created":{"date-parts":[[2018,2,7]],"date-time":"2018-02-07T23:30:44Z","timestamp":1518046244000},"page":"1-9","source":"Crossref","is-referenced-by-count":3,"title":["Experimental Nanopulse Ablation of Multiple Membrane Parasite on Ex Vivo Hydatid Cyst"],"prefix":"10.1155","volume":"2018","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4573-9111","authenticated-orcid":true,"given":"Xinhua","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Hepatobiliary and Pancreatic Surgery, Key Laboratory of Combined Multi-Organ Transplantation, Ministry of Public Health, The First Affiliated Hospital, Zhejiang University, Hangzhou, Zhejiang 310003, 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