{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T14:51:08Z","timestamp":1753887068679,"version":"3.41.2"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,9,13]],"date-time":"2018-09-13T00:00:00Z","timestamp":1536796800000},"content-version":"vor","delay-in-days":255,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002383","name":"King Saud University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002383","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:p><jats:italic>Nepeta deflersiana<\/jats:italic> (Lamiaceae) is a well\u2010known medicinal plant that grows in Saudi Arabia. This plant is used in Saudi and Yemeni folk medicine as an anti\u2010inflammatory, carminative, and antirheumatic agent. In order to prove its use in folk medicine, four different extracts from the aerial parts of the plant: petroleum ether, chloroform, ethyl acetate, and <jats:italic>n<\/jats:italic>\u2010butanol extracts were subjected to biological assays to screen PPAR<jats:italic>\u03b1<\/jats:italic> and PPAR<jats:sub><jats:italic>\u03d2<\/jats:italic><\/jats:sub> agnostic, antioxidant, anti\u2010inflammatory, and cytotoxic activities. Ethyl acetate and <jats:italic>n<\/jats:italic>\u2010butanol extracts of <jats:italic>N. deflersiana<\/jats:italic> NDEE and NDBE, respectively, showed a decrease in oxidative stress and inhibition of both NF\u2010kB and iNOS activities with no cytotoxic effects on four human cancer cell lines. Both active extracts were standardized using two bioactive metabolites which were isolated from the aerial parts of the same plant [8\u2010epi\u20107\u2010deoxyloganic acid (compound <jats:bold>1<\/jats:bold>) and Ursolic acid (compound <jats:bold>2<\/jats:bold>)] by developing a validated HPTLC method. It was found to provide a sharp and compact band of compound <jats:bold>1<\/jats:bold> at R<jats:sub>f<\/jats:sub> = 0.07 and R<jats:sub>f<\/jats:sub> = 0.57 for compound <jats:bold>2<\/jats:bold>, using chloroform, methanol, and formic acid (8.9:0.8:0.3, v\/v\/v) as mobile phase at 550 nm. Compounds <jats:bold>1<\/jats:bold> and <jats:bold>2<\/jats:bold> were found in NDEE by 9.59 %, w\/w, and 84.63 %, w\/w, respectively, and by 11.97 %, w\/w, and 21.26 %, w\/w, respectively, in NDBE.<\/jats:p>","DOI":"10.1155\/2018\/8790769","type":"journal-article","created":{"date-parts":[[2018,9,13]],"date-time":"2018-09-13T23:33:53Z","timestamp":1536881633000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Biological Evaluation of Different Extracts of Aerial Parts of <i>Nepeta deflersiana<\/i> and Standardization of Active Extracts Using 8\u2010Epi\u20107\u2010Deoxyloganic Acid and Ursolic Acid by Validated HPTLC Method"],"prefix":"10.1155","volume":"2018","author":[{"given":"Raha","family":"Orfali","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nasir Ali","family":"Siddiqui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Perwez","family":"Alam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tawfeq Abdullah","family":"Alhowiriny","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Areej Mohammad","family":"Al-Taweel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sami","family":"Al-Yahya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Najwa Mohammed","family":"Majrashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rashad","family":"Mehmood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shabana Iqrar","family":"Khan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3318-9861","authenticated-orcid":false,"given":"Shagufta","family":"Perveen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,9,13]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2004.11.024"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2006.05.004"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2003.12.012"},{"volume-title":"Medicinal Plants of Saudi Arabia","year":"2000","author":"Mossa J.","key":"e_1_2_9_4_2"},{"key":"e_1_2_9_5_2","first-page":"63","article-title":"Studies in the flora of Arabia 2, some new and interesting species of Labiatae","volume":"40","author":"Hedge C.","year":"1982","journal-title":"Notes from the Royal Botanic Garden, Edinburgh"},{"key":"e_1_2_9_6_2","first-page":"189","article-title":"Chemical composition, antimicrobial and antioxidant activities of the essential oil of Nepeta deflersiana growing in Yemen","volume":"6","author":"Mothana R. 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