{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:37:40Z","timestamp":1761676660733,"version":"3.37.3"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Development Project of Shandong Province","award":["2014GSF119022"],"award-info":[{"award-number":["2014GSF119022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p>Deregulation of microRNAs (miRNAs) contributes to nontraumatic osteonecrosis of the femoral head (ONFH-N), but the differentially expressed circulating miRNAs in patients with ONFH-N receiving nonsurgical therapy are unknown. This study aimed to determine the miRNAs expression profile of patients with ONFH-N receiving conservative treatments. This was a case-control prospective study of 43 patients with ONFH-N and 43 participants without ONFH-N, enrolled from 10\/2014 to 10\/2016 at the Department of Orthopedics of the Linyi People\u2019s Hospital (China). The two groups were matched for age, gender, and living area. Microarray analysis and quantitative RT-PCR were used to examine the differentially expressed miRNAs. Bioinformatics was used to predict miRNA target genes and signaling pathways. Microarray and quantitative RT-PCR revealed that nine miRNAs were downregulated and five miRNAs were upregulated in ONFH-N<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mo stretchy=\"false\">(<\/mml:mo><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">3<\/mml:mn><mml:mo stretchy=\"false\">)<\/mml:mo><\/mml:math>compared with controls<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mo stretchy=\"false\">(<\/mml:mo><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">3<\/mml:mn><mml:mo stretchy=\"false\">)<\/mml:mo><\/mml:math>. Bioinformatics showed that calcium-mediated signaling pathway, regulation of calcium ion transmembrane transporter activity, cytoskeletal protein binding, and caveolae macromolecular signaling complex were probably regulated by the identified differentially expressed miRNAs. In the remaining 80 subjects (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">40<\/mml:mn><\/mml:math>\/group), miR-335-5p was downregulated<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mo stretchy=\"false\">(<\/mml:mo><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.01<\/mml:mn><mml:mo stretchy=\"false\">)<\/mml:mo><\/mml:math>and miR-100-5p was upregulated<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mo stretchy=\"false\">(<\/mml:mo><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.02<\/mml:mn><mml:mo stretchy=\"false\">)<\/mml:mo><\/mml:math>in ONFH-N compared with controls. In conclusion, some miRNAs are differentially expressed in conservatively treated ONFH-N compared with controls. Those miRNAs could contribute to the pathogenesis of ONFH-N.<\/jats:p>","DOI":"10.1155\/2018\/9015758","type":"journal-article","created":{"date-parts":[[2018,5,24]],"date-time":"2018-05-24T23:31:50Z","timestamp":1527204710000},"page":"1-8","source":"Crossref","is-referenced-by-count":5,"title":["Differentially Expressed MicroRNAs in Conservatively Treated Nontraumatic Osteonecrosis Compared with Healthy Controls"],"prefix":"10.1155","volume":"2018","author":[{"given":"Biaofang","family":"Wei","sequence":"first","affiliation":[{"name":"Department of Orthopedics, Linyi People\u2019s Hospital, Linyi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, First School of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 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