{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T16:07:14Z","timestamp":1788538034255,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,9,30]],"date-time":"2018-09-30T00:00:00Z","timestamp":1538265600000},"content-version":"vor","delay-in-days":272,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004515","name":"Universiti Kebangsaan Malaysia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004515","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:p><jats:italic>Gynura procumbens<\/jats:italic> (GP) is a medicinal herb that has long been known as anti\u2010inflammatory and antihyperglycaemic. Recently, this herbal extract has been associated with a profertility effect, suggesting its applicability in treating both diabetes and male infertility. In this study, the effects of GP aqueous extract (GPAE) on diabetic rats were investigated through evaluating testes histology and androgen hormone levels as well as the implantation sites of female rats on copulation with the treated male rats. Three dosages of GPAE were used (150, 300, and 450\u2009mg\/kg), and there were three control groups [normal, diabetic, and metformin\u2010treated diabetic]. Testes histology, androgen hormone levels, and number of implantation sites of the GPAE\u2010treated groups matched those of the normal group in contrast to the diabetic and metformin\u2010treated diabetic controls. Sperm proteomics analysis identified 666 proteins, but only 88 were consistently found in all the control and 450\u2010mg\/kg GPAE\u2010treated groups. Four proteins, including cysteine\u2010rich secretory protein 1, carboxylesterase 5A, zona pellucida binding protein, and phosphatidylethanolamine\u2010binding protein 1, were significantly upregulated with GPAE treatment compared with the diabetic control, matching the protein levels of the normal group. These proteins were mainly involved in sperm maturation, sperm capacitation, and sperm\u2010egg interaction, suggesting that GP treatment was able to restore the fertility of male diabetic rats at molecular protein level. In conclusion, GP treatment effectively treats infertility of male diabetic rats, possibly through the upregulation of proteins related to sperm maturation and sperm\u2010egg interaction.<\/jats:p>","DOI":"10.1155\/2018\/9201539","type":"journal-article","created":{"date-parts":[[2018,9,30]],"date-time":"2018-09-30T23:31:54Z","timestamp":1538350314000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["<i>Gynura procumbens<\/i> Improved Fertility of Diabetic Rats: Preliminary Study of Sperm Proteomic"],"prefix":"10.1155","volume":"2018","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0375-4089","authenticated-orcid":false,"given":"Khaidatul Akmar","family":"Kamaruzaman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wan Mohd","family":"Aizat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0877-3150","authenticated-orcid":false,"given":"Mahanem","family":"Mat Noor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,30]]},"reference":[{"key":"e_1_2_9_1_2","first-page":"5","article-title":"2015 Institute for Public Health","author":"National Health & Morbidity Survey (NHMS)","year":"2015","journal-title":"Fact Sheet"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-012-1079-1"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.a.20250"},{"key":"e_1_2_9_4_2","first-page":"171","article-title":"Streptozotocin diabetes and insulin resistance impairment of spermatogenesis in adult rat testis: Central vs local mechanism","volume":"27","author":"Arikawe A. 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