{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T05:56:09Z","timestamp":1777614969352,"version":"3.51.4"},"reference-count":26,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002873","name":"Chulalongkorn University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002873","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Dentistry"],"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p><jats:italic>Aim and Purpose<\/jats:italic>. Biofilms in dental unit waterlines (DUWLs) are extremely difficult to eliminate. Aim of this study is to evaluate the efficacy of a mixture of four D-amino acids on biofilm dispersion in DUWLs.<jats:italic>Materials and Methods<\/jats:italic>. A mixture of four D-amino acids (D-methionine, D-tryptophan, D-leucine, and D-tyrosine, 10 mM each), distilled water (control), and 0.1 M hydrochloric acid (HCl) was used in the experiment. In laboratory, pieces of DUWLs covered with biofilms were submerged in different solutions for 5 days, flushed, and measured OD<jats:sup>600<\/jats:sup>of the dispersed biofilms. Remnants of biofilms on the DUWLs were evaluated by SEM. In clinic, fifteen DCUs were incubated with test and control solutions, flushed, and measured OD<jats:sup>600<\/jats:sup>of the dispersed biofilms. Microbial count of DUWL output water was enumerated twice a week for four weeks.<jats:italic>Results<\/jats:italic>. There was a slight, but not significant, increase in OD<jats:sup>600<\/jats:sup>of flushing water in D-amino acids group. D-amino acids effectively reduced bacterial plaque as demonstrated by SEM. Incubation with D-amino acids significantly reduced biofilms especially after the first day of flushing. Bacterial count in DUWL output water was significantly reduced after treatment with D-amino acids.<jats:italic>Conclusion<\/jats:italic>. D-amino acids are applicable as biofilm dispersing agents in DUWLs.<\/jats:p>","DOI":"10.1155\/2018\/9413925","type":"journal-article","created":{"date-parts":[[2018,1,14]],"date-time":"2018-01-14T18:31:27Z","timestamp":1515954687000},"page":"1-7","source":"Crossref","is-referenced-by-count":23,"title":["Application of D-Amino Acids as Biofilm Dispersing Agent in Dental Unit Waterlines"],"prefix":"10.1155","volume":"2018","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5652-3092","authenticated-orcid":true,"given":"Ruchanee Salingcarnboriboon","family":"Ampornaramveth","sequence":"first","affiliation":[{"name":"Research Unit on Oral Microbiology and Immunology, Department of Microbiology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nilada","family":"Akeatichod","sequence":"additional","affiliation":[{"name":"Research Unit on Oral Microbiology and Immunology, Department of Microbiology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand"},{"name":"Pakhum Hospital, Buriram, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jesita","family":"Lertnukkhid","sequence":"additional","affiliation":[{"name":"Research Unit on Oral Microbiology and Immunology, Department of Microbiology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nichakorn","family":"Songsang","sequence":"additional","affiliation":[{"name":"Research Unit on Oral Microbiology and Immunology, Department of Microbiology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand"},{"name":"Trat Hospital, Trat, 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