{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T11:18:09Z","timestamp":1790680689434,"version":"4.1.0"},"reference-count":55,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,7,9]],"date-time":"2018-07-09T00:00:00Z","timestamp":1531094400000},"content-version":"vor","delay-in-days":189,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81673866"],"award-info":[{"award-number":["81673866"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81602652"],"award-info":[{"award-number":["81602652"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["16411955000"],"award-info":[{"award-number":["16411955000"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:p>Chronic ulcer, including diabetic ulcer, varicose ulcer, and pressure ulcer, negatively affects patients\u2019 quality of life. As microbiology plays an important role in the mechanism of pathology for chronic wound healing, this study concentrates on microecology environment of the wound and how Traditional Chinese Medicine (TCM) regulates wound bacteria. <jats:italic>Method<\/jats:italic>. The study took wound samples from 35 patients and analyzed bacteria variation before and after TCM treatment by 16s rRNA sequencing. All samples were evaluated from aspects of <jats:italic>\u03b1<\/jats:italic>\u2010diversity, <jats:italic>\u03b2<\/jats:italic>\u2010diversity, and Simpson\u2019s Diversity index. <jats:italic>Result<\/jats:italic>. After total DNA extraction, PCR, and 16S rRNA sequencing of wound bacteria from 35 individuals, it was discovered that younger patients with shorter course of disease have a higher microbial diversity and were easier to recover from ulcers. Additionally, gender also played a vital role in wound healing, and a significant microbial diversity existed between male and female patients. <jats:italic>Conclusion<\/jats:italic>. Patients with chronic ulcers achieved a positive effect after TCM treatment (skin\u2010producing ointment). Mechanistically, TCM helped promote wound healing by regulating the wound microbiota.<\/jats:p>","DOI":"10.1155\/2018\/9463295","type":"journal-article","created":{"date-parts":[[2018,7,9]],"date-time":"2018-07-09T23:34:38Z","timestamp":1531179278000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Microbial Diversity of Chronic Wound and Successful Management of Traditional Chinese 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