{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T14:48:02Z","timestamp":1753886882203,"version":"3.41.2"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T00:00:00Z","timestamp":1515542400000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Young Talents Training Program of the Fujian Health Industry","award":["2015-ZQN-ZD-7","2016-1-84"],"award-info":[{"award-number":["2015-ZQN-ZD-7","2016-1-84"]}]},{"name":"Fujian Provincial Health and Family Planning Youth Research Program","award":["2015-ZQN-ZD-7","2016-1-84"],"award-info":[{"award-number":["2015-ZQN-ZD-7","2016-1-84"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:p>Structural changes in symbiotic human microorganisms can affect host phenotype. Liver\u2010fire hyperactivity syndrome (LFHS) presents as bitter taste, halitosis, xerostomia, odontalgia, and other oral symptoms. LFHS is associated with hypertension (EH). In this study, tongue flora was analyzed to further understand the intrinsic relationship between tongue flora and LFHS. Samples of tongue coating, from 16 patients with EH\u2010LFHS, 16 with EH\u2010non\u2010LFHS, and 16 controls, were obtained; then, 16S rRNA variable (V3\u2010V4) regions were amplified and sequenced by MiSeq PE300 Sequencing. Tag clustering and Operational Taxonomic Units (OTUs) abundance analysis were used to compare the OTU sequence with the 16S database. The species were classified, and diversity and structure of the bacterial flora were compared between the three groups. Alpha diversity analysis, including Observed Species index and Chao index, indicated significantly higher richness of species in patients with EH\u2010LFHS (<jats:italic>p<\/jats:italic> &lt; 0.05). Higher phylogenetic diversity, in patients with EH\u2010non\u2010LFHS, indicates greater differences in evolutionary history than in patients with EH\u2010LFHS. <jats:italic>Streptococcus<\/jats:italic>, <jats:italic>Rothia<\/jats:italic>, <jats:italic>Neisseria<\/jats:italic>, and <jats:italic>Sphingomonas<\/jats:italic> were the most prevalent in patients with EH\u2010LFHS, differed from the other two groups. This indicates that richer bacterial diversity, and structure associated with EH\u2010LFHS, may affect the occurrence, development, and outcome of hypertension and syndrome subtypes recognized by TCM.<\/jats:p>","DOI":"10.1155\/2018\/9536924","type":"journal-article","created":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T23:38:22Z","timestamp":1515627502000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Association of Tongue Bacterial Flora and Subtypes of Liver\u2010Fire Hyperactivity Syndrome in Hypertensive Patients"],"prefix":"10.1155","volume":"2018","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4271-4848","authenticated-orcid":false,"given":"Jie-wei","family":"Luo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cong-huai","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao-bin","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing-yu","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong-xi","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-wei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,1,10]]},"reference":[{"key":"e_1_2_9_1_2","article-title":"Ome sweet\u2032 omics - a genealogical treasury of words","volume":"15","author":"Lederberg J.","year":"2001","journal-title":"The Scientist"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.096651.109"},{"key":"e_1_2_9_3_2","article-title":"First comprehensive database of human oral microbiome launched","author":"NIH\/National Institute of Dental and Craniofacial Research","year":"2008","journal-title":"ScienceDaily"},{"volume-title":"Diagnostics of Traditional Chinese Medicine","year":"2013","author":"Wang T. 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