{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T20:12:42Z","timestamp":1790626362623,"version":"4.1.0"},"reference-count":54,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,12,23]],"date-time":"2019-12-23T00:00:00Z","timestamp":1577059200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001826","name":"ZonMw","doi-asserted-by":"publisher","award":["116003005"],"award-info":[{"award-number":["116003005"]}],"id":[{"id":"10.13039\/501100001826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001826","name":"ZonMw","doi-asserted-by":"publisher","award":["016.096.359"],"award-info":[{"award-number":["016.096.359"]}],"id":[{"id":"10.13039\/501100001826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["116003005"],"award-info":[{"award-number":["116003005"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["016.096.359"],"award-info":[{"award-number":["016.096.359"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Stem Cells International"],"published-print":{"date-parts":[[2019,12,23]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Cell-based therapies are being developed to meet the need for curative therapy in chronic kidney disease (CKD). Bone marrow- (BM-) derived mesenchymal stromal cells (MSCs) enhance tissue repair and induce neoangiogenesis through paracrine action of secreted proteins and extracellular vesicles (EVs). Administration of allogeneic BM MSCs is less desirable in a patient population likely to require a kidney transplant, but potency of autologous MSCs should be confirmed, given previous indications that CKD-induced dysfunction is present. While the immunomodulatory capacity of CKD BM MSCs has been established, it is unknown whether CKD affects wound healing and angiogenic potential of MSC-derived CM and EVs. <jats:italic>Methods<\/jats:italic>. MSCs were cultured from BM obtained from kidney transplant recipients (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>N<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>15<\/mml:mn><\/mml:math>) or kidney donors (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>N<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>17<\/mml:mn><\/mml:math>). Passage 3 BM MSCs and BM MSC-conditioned medium (CM) were used for experiments. EVs were isolated from CM by differential ultracentrifugation. BM MSC differentiation capacity, proliferation, and senescence-associated <jats:italic>\u03b2<\/jats:italic>-galactosidase activity was assessed. <jats:italic>In vitro<\/jats:italic> promigratory and proangiogenic capacity of BM MSC-derived CM and EVs was assessed using an <jats:italic>in vitro<\/jats:italic> scratch wound assay and Matrigel angiogenesis assay. <jats:italic>Results<\/jats:italic>. Healthy and CKD BM MSCs exhibited similar differentiation capacity, proliferation, and senescence-associated <jats:italic>\u03b2<\/jats:italic>-galactosidase activity. Scratch wound migration was not significantly different between healthy and CKD MSCs (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.18<\/mml:mn><\/mml:math>). Healthy and CKD BM MSC-derived CM induced similar tubule formation (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.21<\/mml:mn><\/mml:math>). There was also no difference in paracrine regenerative function of EVs (scratch wound: <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.6<\/mml:mn><\/mml:math>; tubulogenesis: <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.46<\/mml:mn><\/mml:math>). <jats:italic>Conclusions<\/jats:italic>. Our results indicate that MSCs have an intrinsic capacity to produce proangiogenic paracrine factors, including EVs, which is not affected by donor health status regarding CKD. This suggests that autologous MSC-based therapy is a viable option in CKD.<\/jats:p>","DOI":"10.1155\/2019\/1232810","type":"journal-article","created":{"date-parts":[[2019,12,23]],"date-time":"2019-12-23T18:30:28Z","timestamp":1577125828000},"page":"1-12","source":"Crossref","is-referenced-by-count":14,"title":["Paracrine Proangiogenic Function of Human Bone Marrow-Derived Mesenchymal Stem Cells Is Not Affected by Chronic Kidney Disease"],"prefix":"10.1155","volume":"2019","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7777-646X","authenticated-orcid":true,"given":"Femke C. C.","family":"van Rhijn-Brouwer","sequence":"first","affiliation":[{"name":"Department of Nephrology and Hypertension, Regenerative Medicine Center Utrecht, UMC Utrecht, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bas W. M.","family":"van Balkom","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension, Regenerative Medicine Center Utrecht, UMC Utrecht, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana A.","family":"Papazova","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension, Regenerative Medicine Center Utrecht, UMC Utrecht, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, Netherlands"},{"name":"Department of Anesthesiology, VU University Medical Center, Boelelaan 1117, 1081 HV, Amsterdam, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di\u00ebnty H. M.","family":"Hazenbrink","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension, Regenerative Medicine Center Utrecht, UMC Utrecht, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anke J.","family":"Meijer","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension, Regenerative Medicine Center Utrecht, UMC Utrecht, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isaac","family":"Brete","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension, Regenerative Medicine Center Utrecht, UMC Utrecht, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vidalmar","family":"Briceno","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension, Regenerative Medicine Center Utrecht, UMC Utrecht, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arjan D.","family":"van Zuilen","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension, Regenerative Medicine Center Utrecht, UMC Utrecht, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raechel J.","family":"Toorop","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery, UMC Utrecht, Utrecht University, Heidelberglaan 100, 3584 CX, Utrecht, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joost O.","family":"Fledderus","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension, Regenerative Medicine Center Utrecht, UMC Utrecht, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, 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