{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T01:54:44Z","timestamp":1772675684733,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,6,12]],"date-time":"2019-06-12T00:00:00Z","timestamp":1560297600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2019,6,12]]},"abstract":"<jats:p>\n                    Toll-like receptors (TLRs) play a key role in the innate immune response to numerous pathogens, including\n                    <jats:italic>Acanthamoeba<\/jats:italic>\n                    spp. The aim of this study was to determine the expression of TLR2 and TLR4 in the eyes of mice following intranasal infection with\n                    <jats:italic>Acanthamoeba<\/jats:italic>\n                    spp. in relation to the host\u2019s immunological status. Amoebae used in this study were isolated from the bronchial aspirate of a patient with acute myeloid leukemia (AML) and atypical symptoms of pneumonia. We found statistically significant differences in the expression of TLR2 and TLR4 in the eye of immunocompetent mice at 8, 16, and 24 days after\n                    <jats:italic>Acanthamoeba<\/jats:italic>\n                    spp. infection (dpi) compared to control group. Immunosuppressed mice showed significant differences in the expression of TLR2 at 16 and 24\u2009dpi compared to uninfected animals. Our results indicate that TLR2 and TLR4 are upregulated in the eyes of mice in response to\n                    <jats:italic>Acanthamoeba<\/jats:italic>\n                    spp. We suggest that it is possible for trophozoites to migrate through the optic nerve from the brain to the eyes. The course of disseminated acanthamoebiasis may be influenced by the host\u2019s immunological status, and the observed changes in expression of TLR2 and TLR4 in the host\u2019s organs may indicate the role of these receptors in the pathomechanism of acanthamoebiasis.\n                  <\/jats:p>","DOI":"10.1155\/2019\/1401894","type":"journal-article","created":{"date-parts":[[2019,6,12]],"date-time":"2019-06-12T19:30:33Z","timestamp":1560367833000},"page":"1-8","source":"Crossref","is-referenced-by-count":11,"title":["Expression of Toll-Like Receptors (TLR2 and TLR4) in the Eyes of Mice with Disseminated Acanthamoebiasis"],"prefix":"10.1155","volume":"2019","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0051-2295","authenticated-orcid":true,"given":"Karolina","family":"Kot","sequence":"first","affiliation":[{"name":"Department of Biology and Medical Parasitology, Pomeranian Medical University in Szczecin, 70-204 Szczecin, 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