{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T11:11:28Z","timestamp":1759057888178,"version":"3.37.3"},"reference-count":22,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,1,17]],"date-time":"2019-01-17T00:00:00Z","timestamp":1547683200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002657","name":"Istanbul \u00dcniversitesi","doi-asserted-by":"publisher","award":["5285","4399"],"award-info":[{"award-number":["5285","4399"]}],"id":[{"id":"10.13039\/501100002657","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002657","name":"Istanbul \u00dcniversitesi","doi-asserted-by":"publisher","award":["5285","4399"],"award-info":[{"award-number":["5285","4399"]}],"id":[{"id":"10.13039\/501100002657","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2019,1,17]]},"abstract":"<jats:p>Epigenetic changes have major role in the normal development and programming of gene expression. Aberrant methylation results in carcinogenesis. The primary objective of our study is to determine whether primary tumor tissue and cultured tumor cells in 2D and 3D tissue culture systems have the same methylation signature for<jats:italic>PAX5<\/jats:italic>,<jats:italic>TMPRSS2<\/jats:italic>, and<jats:italic>SBDS<\/jats:italic>. These findings will play an important role in developing in vitro model system to understand the effect of methylation inhibitors on primary tumor tissue. In a previous study<jats:italic>PAX5<\/jats:italic>,<jats:italic>TMPRSS2<\/jats:italic>, and<jats:italic>SBDS<\/jats:italic>genes that we are investigating were reported to be methylated more than 60% in breast cancer and malignant melanoma cell lines. However, these genes have never been studied in primary tumor tissues. Thus, primary tumor tissues of breast cancer and malignant melanoma were first grown in 2D and 3D cultures. Then these two types of tumor tissues and their 2D and 3D cultures were investigated for changes considering methylation levels in<jats:italic>PAX5<\/jats:italic>,<jats:italic>TMPRSS2<\/jats:italic>, and<jats:italic>SBDS<\/jats:italic>genes using real-time polymerase chain reaction. No differences were observed in the primary tissues and culture systems for both<jats:italic>PAX5<\/jats:italic>and<jats:italic>TMPRSS2<\/jats:italic>in malignant melanoma tissues. We found that<jats:italic>PAX5<\/jats:italic>gene was an efficient marker to measure the effects of methylation inhibitors for in vitro systems for malignant melanoma tissue.<\/jats:p>","DOI":"10.1155\/2019\/1407167","type":"journal-article","created":{"date-parts":[[2019,1,17]],"date-time":"2019-01-17T23:30:41Z","timestamp":1547767841000},"page":"1-9","source":"Crossref","is-referenced-by-count":3,"title":["Methylation Changes of Primary Tumors, Monolayer, and Spheroid Tissue Culture Environments in Malignant Melanoma and Breast Carcinoma"],"prefix":"10.1155","volume":"2019","author":[{"given":"\u00d6zge","family":"\u015e\u00fckr\u00fco\u011flu Erdo\u011fan","sequence":"first","affiliation":[{"name":"Istanbul University, Institute of Oncology, Department of Basic Oncology, Cancer Genetics Division, Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seda","family":"K\u0131l\u0131\u00e7 Erciyas","sequence":"additional","affiliation":[{"name":"Istanbul University, Institute of Oncology, Department of Basic Oncology, Cancer Genetics Division, Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayhan","family":"Bilir","sequence":"additional","affiliation":[{"name":"Istanbul Aydin University, Faculty of Medicine, Department of Histology and Embryology, Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u015eeref","family":"Bu\u011fra Tun\u00e7er","sequence":"additional","affiliation":[{"name":"Istanbul University, Institute of Oncology, Department of Basic Oncology, Cancer Genetics Division, Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Demet","family":"Akdeniz \u00d6demi\u015f","sequence":"additional","affiliation":[{"name":"Istanbul University, Institute of Oncology, Department of Basic Oncology, Cancer Genetics Division, Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u0131d\u0131ka","family":"Kurul","sequence":"additional","affiliation":[{"name":"Istanbul University, Institute of Oncology, Department of Clinical Oncology, Surgical Unit, Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hasan","family":"Karanl\u0131k","sequence":"additional","affiliation":[{"name":"Istanbul University, Institute of Oncology, Department of Clinical Oncology, Surgical Unit, Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neslihan","family":"Cab\u0131o\u011flu","sequence":"additional","affiliation":[{"name":"Istanbul University, Istanbul Faculty of Medicine, Department of Surgery, Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8919-0482","authenticated-orcid":true,"given":"H\u00fclya","family":"Yaz\u0131c\u0131","sequence":"additional","affiliation":[{"name":"Istanbul University, Institute of Oncology, Department of Basic Oncology, Cancer Genetics Division, Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25516"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.12492"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1186\/s12920-015-0147-4"},{"issue":"44","key":"4","doi-asserted-by":"crossref","first-page":"78163","DOI":"10.18632\/oncotarget.18293","volume":"8","year":"2017","journal-title":"Oncotarget"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2017.10.006"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.16254"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.3390\/cancers10080268"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1038\/nature12981"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1172\/JCI33295"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.27423"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0749.2007.00367.x"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0712251105"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1038\/44076"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.3121\/cmr.2010.891"},{"issue":"17","key":"15","first-page":"4180","volume":"59","year":"1999","journal-title":"Cancer Research"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M105044200"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-02-075184"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M414656200"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1080\/13543776.2016.1209488"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1007\/s10911-016-9350-y"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.13.e65"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl1134"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2019\/1407167.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2019\/1407167.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2019\/1407167.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,22]],"date-time":"2020-11-22T21:16:42Z","timestamp":1606079802000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/bmri\/2019\/1407167\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,17]]},"references-count":22,"alternative-id":["1407167","1407167"],"URL":"https:\/\/doi.org\/10.1155\/2019\/1407167","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"type":"print","value":"2314-6133"},{"type":"electronic","value":"2314-6141"}],"subject":[],"published":{"date-parts":[[2019,1,17]]}}}