{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T19:15:33Z","timestamp":1779909333786,"version":"3.53.1"},"reference-count":44,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,11,12]],"date-time":"2019-11-12T00:00:00Z","timestamp":1573516800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2019,11,12]]},"abstract":"<jats:p>The literature shows that phenolic compounds possess important antioxidant and anti-inflammatory activities; however, the mechanism underlying these effects is not elucidated yet. The genus<jats:italic>Calea<\/jats:italic>is used in folk medicine to treat rheumatism, respiratory diseases, and digestive problems. In this context, some phenolic compounds were isolated with high purity from<jats:italic>Calea uniflora<\/jats:italic>Less. and identified as noreugenin (NRG) and<jats:italic>\u03b1<\/jats:italic>-hydroxy-butein (AH-BU). The aim of this study was to analyze the effect of these compounds on cell viability, the activity of myeloperoxidase (MPO), and apoptosis of mouse neutrophils using<jats:italic>ex vivo<\/jats:italic>tests. Furthermore, the effect of these compounds on the cytokines, interleukin 1 beta (IL-1<jats:italic>\u03b2<\/jats:italic>), interleukin 17A (IL-17A), and interleukin 10 (IL-10), and oxidative stress was investigated by analyzing lipid peroxidation (the concentration of thiobarbituric acid reactive substances (TBARS)) and activities of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST), using a murine model of neutrophilic inflammation. The NRG and AH-BU reduce MPO activity and increase neutrophil apoptosis (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>). These compounds reduced the generation of oxygen reactive species and IL-1<jats:italic>\u03b2<\/jats:italic>and IL-17A levels but increased IL-10 levels (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>). This study demonstrated that NRG and AH-BU show a significant anti-inflammatory effect by inhibiting the MPO activity and increasing neutrophil apoptosis in primary cultures of mouse neutrophils. These effects were at least partially associated with blocking reactive species generation, inhibiting IL-1<jats:italic>\u03b2<\/jats:italic>and IL-17A, and increasing IL-10 levels.<\/jats:p>","DOI":"10.1155\/2019\/1468502","type":"journal-article","created":{"date-parts":[[2019,11,12]],"date-time":"2019-11-12T23:30:25Z","timestamp":1573601425000},"page":"1-10","source":"Crossref","is-referenced-by-count":7,"title":["Phenolic Compounds Isolated from<i>Calea uniflora<\/i>Less. Promote Anti-Inflammatory and Antioxidant Effects in Mice Neutrophils (<i>Ex Vivo<\/i>) and in Mice Pleurisy Model (<i>In Vivo<\/i>)"],"prefix":"10.1155","volume":"2019","author":[{"given":"Julia Salvan","family":"da Rosa","sequence":"first","affiliation":[{"name":"Graduate Course of Pharmacy, Center of Health Sciences, Federal University of Santa Catarina (UFSC), Florian\u00f3polis, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus Vinicius Pereira Dos Santos","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Graduate Course of Pharmacy, Center of Health Sciences, Federal University of Santa Catarina (UFSC), Florian\u00f3polis, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo Benedetti","family":"Parisotto","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Center of Biological Sciences, Federal University of Santa Catarina (UFSC), Florian\u00f3polis, SC, 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