{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T03:56:10Z","timestamp":1787630170270,"version":"build-2736575974"},"reference-count":44,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,10,28]],"date-time":"2019-10-28T00:00:00Z","timestamp":1572220800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["Nr 2013\/11\/B\/NZ5\/03298"],"award-info":[{"award-number":["Nr 2013\/11\/B\/NZ5\/03298"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Diabetes Research"],"published-print":{"date-parts":[[2019,10,28]]},"abstract":"<jats:p><jats:italic>Background and Aims<\/jats:italic>. Diabetic foot ulcers (DFUs) are linked to amputations and premature deaths. Negative pressure wound therapy (NPWT) has been used for DFUs. The mechanism of NPWT\u2019s action may be associated with its influence on circulating molecules. We assessed NPWT\u2019s effect on the plasma levels of angiopoietin-2 (Ang2), a key regulator of angiogenesis, and its microvesicular receptors (Tie2) as well as the microvesicles (MVs) themselves in DFU patients. <jats:italic>Materials and Methods<\/jats:italic>. We included 69 patients with type 2 diabetes mellitus (T2DM) and neuropathic, noninfected DFUs\u201449 were treated with NPWT and 20 were treated with standard therapy (ST). Assigning patients to the NPWT group was not random but based on DFU characteristics, especially wound area. Ang2 was measured by ELISA in the entire group, while in a subgroup of 19 individuals on NPWT and 10 on ST, flow cytometry was used to measure Tie2+ and the corresponding isotype control (Iso+) and annexin V (AnnV+) as well as total MVs. Measurements were performed at the beginning and after <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mn>8<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn>1<\/mml:mn><mml:mtext>\u2009<\/mml:mtext><mml:mtext>days<\/mml:mtext><\/mml:math> of therapy. <jats:italic>Results<\/jats:italic>. Treatment groups were similar for basic characteristics but differed by their median DFU areas (10.3 (4.2-18.9) vs. 1.3 (0.9-3.4)\u00a0cm<jats:sup>2<\/jats:sup>, <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>p<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.0001<\/mml:mn><\/mml:math>). At day 0, no difference was observed in Ang2 levels, total MVs, MV Tie+, and MV AnnV+ between the groups. Ang2 decreased after 8 days in the NPWT group, unlike in the ST group (3.54 (2.40-5.40) vs. 3.32 (2.33-4.61), <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>p<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.02<\/mml:mn><\/mml:math>, and <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mn>3.19<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn>1.11<\/mml:mn><\/mml:math> vs. <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mn>3.19<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn>1.29<\/mml:mn><mml:mtext>\u2009<\/mml:mtext><mml:mtext>ng<\/mml:mtext><mml:mo>\/<\/mml:mo><mml:mtext>mL<\/mml:mtext><\/mml:math>, <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\"><mml:mi>p<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.98<\/mml:mn><\/mml:math>, respectively). No other parameters were identified that may have been influenced by the NPWT treatment. <jats:italic>Conclusion<\/jats:italic>. NPWT in T2DM patients with neuropathic, noninfected DFU seems to lead to reduction of the Ang2 level. Influencing the level of Ang2 may constitute one of NPWT-related mechanisms to accelerate wound healing.<\/jats:p>","DOI":"10.1155\/2019\/1756798","type":"journal-article","created":{"date-parts":[[2019,10,28]],"date-time":"2019-10-28T19:30:53Z","timestamp":1572291053000},"page":"1-7","source":"Crossref","is-referenced-by-count":9,"title":["Effects of Negative Pressure Wound Therapy on Levels of Angiopoetin-2 and Other Selected Circulating Signaling Molecules in Patients with Diabetic Foot Ulcer"],"prefix":"10.1155","volume":"2019","author":[{"given":"Jerzy","family":"Hohendorff","sequence":"first","affiliation":[{"name":"Department of Metabolic Diseases, Jagiellonian University Medical College, Krakow, Poland"},{"name":"Clinic of Metabolic Diseases, University Hospital, Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3999-6725","authenticated-orcid":true,"given":"Anna","family":"Drozdz","sequence":"additional","affiliation":[{"name":"Department of Medical Physics, Marian Smoluchowski Institute of Physics, Jagiellonian University, Krakow, Poland"},{"name":"Malopolska Center of Biotechnology, Jagiellonian University, Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Borys","sequence":"additional","affiliation":[{"name":"Department of Metabolic Diseases, Jagiellonian University Medical College, Krakow, Poland"},{"name":"Clinic of Metabolic Diseases, University Hospital, Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka H.","family":"Ludwig-Slomczynska","sequence":"additional","affiliation":[{"name":"Center for Medical Genomics OMICRON, Jagiellonian University Medical College, Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beata","family":"Kiec-Wilk","sequence":"additional","affiliation":[{"name":"Department of Metabolic Diseases, Jagiellonian University Medical College, Krakow, Poland"},{"name":"Clinic of Metabolic Diseases, University Hospital, Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3589-1715","authenticated-orcid":true,"given":"Ewa L.","family":"Stepien","sequence":"additional","affiliation":[{"name":"Department of Medical Physics, Marian Smoluchowski Institute of Physics, Jagiellonian University, Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4794-0820","authenticated-orcid":true,"given":"Maciej T.","family":"Malecki","sequence":"additional","affiliation":[{"name":"Department of Metabolic Diseases, Jagiellonian University Medical College, Krakow, Poland"},{"name":"Clinic of Metabolic Diseases, 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Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1615439"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1111\/dme.13054"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1177\/1479164115579005"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1007\/s00592-018-1223-y"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1530\/ec-19-0373"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2018.01.018"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-019-1152-x"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1177\/1534734618794856"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.arcmed.2012.01.006"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2012.04650.x"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000036596.59665.C6"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1111\/apha.12896"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2016.04.025"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2015.09.013"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1080\/21541248.2016.1215283"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.7150\/thno.23334"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0115316"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a006650"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5322.55"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.3390\/cells8050471"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2639"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.172161899"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.10442"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1096\/fj.04-2209fje"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2840-10-55"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1111\/eci.13067"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1002\/dmrr.2700"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1177\/1708538119836360"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2018.02.003"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2011.02.005"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1080\/07853890701779586"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1159\/000488826"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.19082\/5031"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.2337\/db19-637-P"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3780244"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.9636"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0804543106"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.12672"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2007.0400"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.2337\/dc07-2196"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)67695-7"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18050956"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-018-0058-5"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2016.09.009"}],"container-title":["Journal 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