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They home to sites of neovascularization and contribute to angiogenesis by production of paracrine factors. The number and function of proangiogenic cells are impaired in patients with diabetes or cardiovascular diseases. Both conditions can be accompanied by decreased levels of heme oxygenase-1 (HMOX1), cytoprotective, heme-degrading enzyme. Our study is aimed at investigating whether precursors of myeloid angiogenic cells (PACs) treated with known pharmaceuticals would produce media with better proangiogenic activity <jats:italic>in vitro<\/jats:italic> and if such media can be used to stimulate blood vessel growth <jats:italic>in vivo<\/jats:italic>. We used G-CSF-mobilized CD34<jats:sup>+<\/jats:sup> HSPCs, FACS-sorted from healthy donor peripheral blood mononuclear cells (PBMCs). Sorted cells were predominantly CD133<jats:sup>+<\/jats:sup>. CD34<jats:sup>+<\/jats:sup> cells after six days in culture were stimulated with atorvastatin (AT), acetylsalicylic acid (ASA), sulforaphane (SR), resveratrol (RV), or metformin (Met) for 48\u2009h. Conditioned media from such cells were then used to stimulate human aortic endothelial cells (HAoECs) to enhance tube-like structure formation in a Matrigel assay. The only stimulant that enhanced PAC paracrine angiogenic activity was atorvastatin, which also had ability to stabilize endothelial tubes <jats:italic>in vitro<\/jats:italic>. On the other hand, the only one that induced heme oxygenase-1 expression was sulforaphane, a known activator of a HMOX1 inducer\u2014NRF2. None of the stimulants changed significantly the levels of 30 cytokines and growth factors tested with the multiplex test. Then, we used atorvastatin-stimulated cells or conditioned media from them in the Matrigel plug <jats:italic>in vivo<\/jats:italic> angiogenic assay. Neither AT alone in control media nor conditioned media nor AT-stimulated cells affected numbers of endothelial cells in the plug or plug\u2019s vascularization. Concluding, high concentrations of atorvastatin stabilize tubes and enhance the paracrine angiogenic activity of human PAC cells <jats:italic>in vitro<\/jats:italic>. However, the effect was not observed <jats:italic>in vivo<\/jats:italic>. Therefore, the use of conditioned media from atorvastatin-treated PAC is not a promising therapeutic strategy to enhance angiogenesis.<\/jats:p>","DOI":"10.1155\/2019\/1868170","type":"journal-article","created":{"date-parts":[[2019,7,16]],"date-time":"2019-07-16T19:30:33Z","timestamp":1563305433000},"page":"1-15","source":"Crossref","is-referenced-by-count":7,"title":["Atorvastatin and Conditioned Media from Atorvastatin-Treated Human Hematopoietic Stem\/Progenitor-Derived Cells Show Proangiogenic Activity <i>In Vitro<\/i> but Not <i>In Vivo<\/i>"],"prefix":"10.1155","volume":"2019","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1526-3511","authenticated-orcid":true,"given":"Witold N.","family":"Nowak","sequence":"first","affiliation":[{"name":"Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 31-007 Krak\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hevidar","family":"Taha","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 31-007 Krak\u00f3w, Poland"},{"name":"Basic Science Department, College of Agriculture, University of Duhok, Zakho Street 38, 1006 AJ Duhok, Kurdistan Region, Iraq"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4168-9049","authenticated-orcid":true,"given":"Joanna","family":"Markiewicz","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 31-007 Krak\u00f3w, Poland"},{"name":"Bionanoscience and Biochemistry Laboratory, Malopolska Centre of Biotechnology, Jagiellonian University, 30-387 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neli","family":"Kachamakova-Trojanowska","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 31-007 Krak\u00f3w, Poland"},{"name":"International Associated Laboratory, Malopolska Centre for Biotechnology, Jagiellonian University, 30-387 Krak\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacek","family":"St\u0119pniewski","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 31-007 Krak\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Damian","family":"Kl\u00f3ska","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 31-007 Krak\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Urszula","family":"Florczyk-Soluch","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 31-007 Krak\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafa\u0142","family":"Ni\u017cankowski","sequence":"additional","affiliation":[{"name":"2nd Department of Internal Medicine, Jagiellonian University Medical College, Skawi\u0144ska 8, 31-066 Krak\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marzena","family":"Fro\u0142ow","sequence":"additional","affiliation":[{"name":"2nd Department of Internal Medicine, Jagiellonian University Medical College, Skawi\u0144ska 8, 31-066 Krak\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zbigniew","family":"Walter","sequence":"additional","affiliation":[{"name":"Clinics of Hematology, University Hospital, Jagiellonian University Medical College, M. 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