{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:16:30Z","timestamp":1767165390756,"version":"build-2238731810"},"reference-count":30,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,1,27]],"date-time":"2019-01-27T00:00:00Z","timestamp":1548547200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2019,1,27]]},"abstract":"<jats:p>\n                    Postmenopausal osteoporosis (PMOP), as well as its associated increased risk for fragility fracture, is one of the most disabling consequences of aging in women. This present study aimed to identify candidate genes that involve pathogenesis of PMOP and the therapeutic mechanism of Liuweidihuang (LWDH) pills on PMOP. We integrated microarray datasets of PMOP derived from the Gene Expression Omnibus (GEO) to screen differentially expressed genes (DEGs) between PMOP and normal controls as well as patients with PMOP and patients after treatment of LWDH pills. GO and KEGG enrichment analysis for DEGs were performed. The shared DEGs, associated with both the pathogenesis of PMOP and the therapeutic mechanism of LWDH, were further analyzed by protein-protein interaction (PPI) network. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to verify the DEGs obtained by our integrated analysis. Compared with normal controls, 1732 DEGs in PMOP were obtained with\n                    <jats:italic>p<\/jats:italic>\n                    &lt;0.05. According to the qRT-PCR results, expression of ATF2, FBXW7, RDX, and RBBP4 was consistent with that in our integrated analysis, generally. GO and KEGG enrichment analysis showed that those DEGs were significantly enriched in regulation of transcription, DNA-dependent, cytoplasm, protein binding, and MAPK signaling pathway. A total of 58 shared DEGs in PMOP versus normal control and in patients with PMOP versus patients after LWDH treatment were identified, which had opposite expression trend in these two comparisons. In the PPI network, CSNK2A1, ATF2, and FBXW7 were three hub proteins. Three genes including ATF2, FBXW7, and RDX were speculated to be therapeutic targets of LWDH for PMOP based on BATMAN-TCM database. We speculated that three genes of ATF2, FBXW7, and RDX may play crucial roles in both pathogenesis of PMOP and therapeutic mechanism of LWDH on PMOP. Our results may provide clues for the molecular pathogenesis of PMOP and offer new possibilities for treatment of PMOP.\n                  <\/jats:p>","DOI":"10.1155\/2019\/1907906","type":"journal-article","created":{"date-parts":[[2019,1,27]],"date-time":"2019-01-27T18:30:39Z","timestamp":1548613839000},"page":"1-10","source":"Crossref","is-referenced-by-count":9,"title":["Bioinformatics Analysis Reveals the Altered Gene Expression of Patients with Postmenopausal Osteoporosis Using Liuweidihuang Pills Treatment"],"prefix":"10.1155","volume":"2019","author":[{"given":"Rui","family":"Gong","sequence":"first","affiliation":[{"name":"Hebei Medical University Endocrine Teaching and Research Section, China"},{"name":"Department of Gerontology, The Third Hospital of Shijiazhuang, China"},{"name":"Department of Endocrinology, Third Affiliated Hospital of Hebei Medical University, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of ICU, Hebei General Hospital, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Menghui","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery, The Third Hospital of Shijiazhuang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanli","family":"Wang","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology, The Third Hospital of Shijiazhuang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoliang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery, The Third Hospital of Shijiazhuang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijuan","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, The Third Hospital of Shijiazhuang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cuizhao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Laboratory, The Third Hospital of Shijiazhuang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruihong","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Laboratory, The Third Hospital of Shijiazhuang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7440-672X","authenticated-orcid":true,"given":"Yukun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Third Affiliated Hospital of Hebei Medical University, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","first-page":"7","volume":"0981","year":"2015","journal-title":"Experimental & Therapeutic 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