{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T17:43:30Z","timestamp":1740159810589,"version":"3.37.3"},"reference-count":15,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,3,3]],"date-time":"2019-03-03T00:00:00Z","timestamp":1551571200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurology Research International"],"published-print":{"date-parts":[[2019,3,3]]},"abstract":"<jats:p>The human acetylcholine receptor (AChR) is well characterized as the target antigen in myasthenia gravis (MG). Pathogenic antibody responses against the AChR alpha-chain have been investigated extensively and are of diagnostic and prognostic value. However, less is known on the pathogenetic relevance of T-cell responses against epitopes of the different AChR chains (alpha, epsilon, gamma). Using an enzyme-linked immunospot (ELISPOT) assay we measured T-cell responses against recombinant fragments and synthetic peptides of the <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:mi>\u03b1<\/mml:mi><\/mml:mrow><\/mml:math> and the <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mrow><mml:mi>\u03b5<\/mml:mi><\/mml:mrow><\/mml:math> subunits of the human AChR in MG patients (n=15) and in healthy donors (HD; n=9). In MG, highest T-cell responses were noted against recombinantly expressed Epsilon 1-221. Among the synthetic peptides Epsilon 201-215 showed the most prominent T-cell response and represented the peptide with the most remarkable difference between MG and HD. Taken together, prominent T-cell responses against the <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mrow><mml:mi>\u03b5<\/mml:mi><\/mml:mrow><\/mml:math> subunit of the human AChR indicate an important role in the pathogenesis of MG.<\/jats:p>","DOI":"10.1155\/2019\/1969068","type":"journal-article","created":{"date-parts":[[2019,3,3]],"date-time":"2019-03-03T18:31:45Z","timestamp":1551637905000},"page":"1-5","source":"Crossref","is-referenced-by-count":1,"title":["Prominent T-Cell Responses against the Acetylcholine Receptor <i>\u03b5<\/i> Subunit in Myasthenia Gravis"],"prefix":"10.1155","volume":"2019","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5496-2243","authenticated-orcid":true,"given":"Oliver","family":"Neuhaus","sequence":"first","affiliation":[{"name":"Department of Neurology, Medical University of Graz, Graz, Austria"},{"name":"Department of Neurology, SRH Kliniken Landkreis Sigmaringen GmbH, Sigmaringen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karl-Heinz","family":"Wiesm\u00fcller","sequence":"additional","affiliation":[{"name":"EMC Microcollections GmbH, T\u00fcbingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans-Peter","family":"Hartung","sequence":"additional","affiliation":[{"name":"Department of Neurology, Medical University of Graz, Graz, Austria"},{"name":"Department of Neurology, University of D\u00fcsseldorf, D\u00fcsseldorf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heinz","family":"Wiendl","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of T\u00fcbingen, T\u00fcbingen, Germany"},{"name":"Department of Neurology, University of M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1996.0539u.x"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2004.09.017"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(96)10022-0"},{"key":"4","first-page":"481","volume":"17","year":"1997","journal-title":"Critical Reviews in Immunology"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(97)00147-1"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.50.4.1045"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1002\/1531-8249(199902)45:2<224::AID-ANA13>3.0.CO;2-B"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2004.03.019"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/0960-5428(94)00040-U"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(98)00146-5"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2008.06.016"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1993.tb22882.x"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.221220998"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/12.6.747"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/124.4.705"}],"container-title":["Neurology Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/nri\/2019\/1969068.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/nri\/2019\/1969068.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/nri\/2019\/1969068.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,3,3]],"date-time":"2019-03-03T18:31:47Z","timestamp":1551637907000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/nri\/2019\/1969068\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,3]]},"references-count":15,"alternative-id":["1969068","1969068"],"URL":"https:\/\/doi.org\/10.1155\/2019\/1969068","relation":{},"ISSN":["2090-1852","2090-1860"],"issn-type":[{"type":"print","value":"2090-1852"},{"type":"electronic","value":"2090-1860"}],"subject":[],"published":{"date-parts":[[2019,3,3]]}}}