{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T02:09:06Z","timestamp":1777428546452,"version":"3.51.4"},"reference-count":48,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,3,13]],"date-time":"2019-03-13T00:00:00Z","timestamp":1552435200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Collaborative Research Program of the International Centre for Genetic Engineering Biotechnology","award":["CRP\/MOR09-01"],"award-info":[{"award-number":["CRP\/MOR09-01"]}]},{"name":"Collaborative Research Program of the International Centre for Genetic Engineering Biotechnology","award":["CNRST\/I002\/033"],"award-info":[{"award-number":["CNRST\/I002\/033"]}]},{"name":"Molecular Basis of Hemoglobinopathies in Morocco","award":["CRP\/MOR09-01"],"award-info":[{"award-number":["CRP\/MOR09-01"]}]},{"name":"Molecular Basis of Hemoglobinopathies in Morocco","award":["CNRST\/I002\/033"],"award-info":[{"award-number":["CNRST\/I002\/033"]}]},{"name":"Excellence Program of Research of the National Center of Research in Science and Techniques in Morocco","award":["CRP\/MOR09-01"],"award-info":[{"award-number":["CRP\/MOR09-01"]}]},{"name":"Excellence Program of Research of the National Center of Research in Science and Techniques in Morocco","award":["CNRST\/I002\/033"],"award-info":[{"award-number":["CNRST\/I002\/033"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2019,3,13]]},"abstract":"<jats:p>Unlike the other hemoglobinopathies, few researches have been published concerning<jats:italic>\u03b1<\/jats:italic>-thalassemia in Morocco. The epidemiological features and the mutation spectrum of this disease are still unknown. This regional newborn screening is the first to study<jats:italic>\u03b1<\/jats:italic>-thalassemia in the north of Morocco. During the period from January 2015 to December 2016, 1658 newborns umbilical blood samples were investigated. Suspected newborns were screened for<jats:italic>\u03b1<\/jats:italic>-globin defects using Gap-PCR and Multiplex Ligation-dependent Probe Amplification technique. The prevalence of<jats:italic>\u03b1<\/jats:italic>-thalassemia, its mutation spectrum, and its allelic frequencies were described for the first time in Morocco. Six different<jats:italic>\u03b1<\/jats:italic>-globin genetic disorders were detected in 16 neonates. This screening valued the prevalence of<jats:italic>\u03b1<\/jats:italic>-thalassemia in the studied population at 0.96% and showed the wide mutation spectrum and the heterogeneous geographical distribution of the disease. A high rate of carriers was observed in Laouamra, a rural commune in Larache province. Heterogeneity of<jats:italic>\u03b1<\/jats:italic>-globin alleles in Morocco explains the high variability of<jats:italic>\u03b1<\/jats:italic>-thalassemia severity. This diversity reflects the anthropological history of the country. These results would contribute to the prevention of thalassemia in Morocco directing the design of a nationwide screening strategy and awareness campaign.<\/jats:p>","DOI":"10.1155\/2019\/2080352","type":"journal-article","created":{"date-parts":[[2019,3,13]],"date-time":"2019-03-13T23:30:41Z","timestamp":1552519841000},"page":"1-7","source":"Crossref","is-referenced-by-count":13,"title":["Alpha-Thalassemia in North Morocco: Prevalence and Molecular Spectrum"],"prefix":"10.1155","volume":"2019","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7316-700X","authenticated-orcid":true,"given":"Achraf","family":"Laghmich","sequence":"first","affiliation":[{"name":"Biomedical Genomics and Oncogenetics Research Laboratory, Faculty of Sciences and Techniques of Tangier, University Abdelmalek Essa\u00e2di, Tangier 90000, Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4691-0272","authenticated-orcid":true,"given":"Fatima Zahra","family":"Alaoui Ismaili","sequence":"additional","affiliation":[{"name":"Biomedical Genomics and Oncogenetics Research Laboratory, Faculty of Sciences and Techniques of Tangier, University Abdelmalek Essa\u00e2di, Tangier 90000, Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amina","family":"Barakat","sequence":"additional","affiliation":[{"name":"Biomedical Genomics and Oncogenetics Research Laboratory, Faculty of Sciences and Techniques of Tangier, University Abdelmalek Essa\u00e2di, Tangier 90000, Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naima","family":"Ghailani Nourouti","sequence":"additional","affiliation":[{"name":"Biomedical Genomics and Oncogenetics Research Laboratory, Faculty of Sciences and Techniques of Tangier, University Abdelmalek Essa\u00e2di, Tangier 90000, Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed","family":"Khattab","sequence":"additional","affiliation":[{"name":"Pediatric Hemato-Oncology Service, Children\u2019s Hospital, CHU Rabat 10010, Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohcine","family":"Bennani Mechita","sequence":"additional","affiliation":[{"name":"Biomedical Genomics and Oncogenetics Research Laboratory, Faculty of Sciences and Techniques of Tangier, University Abdelmalek Essa\u00e2di, Tangier 90000, Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2010.05572.x"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1038\/35066048"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1172-5-13"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1097\/GIM.0b013e3181fcb468"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.4238\/vol8-2gmr601"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12585"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(13)70150-5"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.21883"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1038\/321744a0"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.12980\/apjtd.7.2017D7-55"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.9790\/3008-09626167"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/S0929-693X(03)00294-X"},{"key":"14","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1100\/tsw.2009.69","volume":"9","year":"2009","journal-title":"Scientific World Journal"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1080\/03630260500454550"},{"issue":"9","key":"16","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1093\/clinchem\/45.9.1495","volume":"45","year":"1999","journal-title":"Clinical Chemistry"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1993.tb04639.x"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.2005.033597"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqx047"},{"issue":"5","key":"20","first-page":"627","volume":"130","year":"2009","journal-title":"Indian Journal of Medical Research"},{"key":"21","first-page":"83","volume":"12","year":"2006","journal-title":"Antropo"},{"key":"22","first-page":"35","volume":"32","year":"2014","journal-title":"Antropo"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1007\/s12041-008-0036-0"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1159\/000207187"},{"issue":"4","key":"25","first-page":"249","volume":"28","year":"1986","journal-title":"Nouvelle revue francaise d\"hematologie"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1159\/000207641"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1975.tb01816.x"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1982.tb07303.x"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-010-1137-4"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1186\/s13023-017-0643-z"},{"key":"31","first-page":"276","volume":"6","year":"2016","journal-title":"Indian Journal of Applied Researc"},{"issue":"3","key":"32","first-page":"233","volume":"40","year":"2015","journal-title":"Iranian Journal of Medical Sciences"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.4084\/mjhid.2013.002"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2257.2006.00792.x"},{"issue":"4","key":"35","first-page":"255","volume":"8","year":"2002","journal-title":"Hong Kong Medical Journal"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.5144\/0256-4947.2013.18"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1081\/HEM-120016372"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1080\/03630260802004301"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1007\/s12687-012-0127-8"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.3109\/03630260008997525"},{"key":"41","volume":"1, article no 23","year":"2001","journal-title":"Bahrain Medical Bulletin"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1111\/j.1447-0756.2005.00333.x"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1590\/1678-4685-gmb-2016-0330"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.3109\/03630260903571286"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1080\/03630260802626053"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1159\/000040863"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.10385"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1089\/gtmb.2008.0123"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-05080-0"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2019\/2080352.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2019\/2080352.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2019\/2080352.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,30]],"date-time":"2020-11-30T14:43:22Z","timestamp":1606747402000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/bmri\/2019\/2080352\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,13]]},"references-count":48,"alternative-id":["2080352","2080352"],"URL":"https:\/\/doi.org\/10.1155\/2019\/2080352","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,13]]}}}