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Plasmids containing the<jats:italic>TRPC6<\/jats:italic>promoter region (with the \u2212254C or G allele) were constructed and then transfected into human embryonic kidney (HEK) 293T cells and human podocytes. Luciferase assays were used to test the promoter activity in both cell lines with or without tumor necrosis factor-<jats:italic>\u03b1<\/jats:italic>(TNF-<jats:italic>\u03b1<\/jats:italic>) treatment, and chromatin immunoprecipitation-polymerase chain reaction (ChIP-PCR) analysis was used to verify the transcription factor that could bind to this mutant sequence. Luciferase results indicate that the activity of the mutant promoter was greater than that of the normal promoter of the<jats:italic>TRPC6<\/jats:italic>gene in both cell lines. We further predicted and verified that this variation was mediated by the nuclear factor kappa B (NF-<jats:italic>\u03ba<\/jats:italic>B) subunit RELA, and TNF-<jats:italic>\u03b1<\/jats:italic>significantly enhanced the transcription activity of<jats:italic>TRPC6<\/jats:italic>with the \u2212254G allele. In conclusion, the \u2212254C&gt;G SNP is a gain-of-function variation of the<jats:italic>TRPC6<\/jats:italic>gene, and it is also an early and effective factor for predicting steroid-resistant nephrotic syndrome (SRNS) in Chinese children.<\/jats:p>","DOI":"10.1155\/2019\/2197837","type":"journal-article","created":{"date-parts":[[2019,6,10]],"date-time":"2019-06-10T23:30:37Z","timestamp":1560209437000},"page":"1-7","source":"Crossref","is-referenced-by-count":2,"title":["\u2212254C&gt;G SNP in the<i>TRPC6<\/i>Gene Promoter Influences Its Expression via Interaction with the NF-<i>\u03ba<\/i>B Subunit RELA in Steroid-Resistant Nephrotic Syndrome Children"],"prefix":"10.1155","volume":"2019","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0528-9136","authenticated-orcid":true,"given":"Xinyu","family":"Kuang","sequence":"first","affiliation":[{"name":"Department of Nephrology and Rheumatology, Shanghai Children\u2019s Hospital, Children\u2019s Hospital of Shanghai Jiao Tong 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